MAP4

MAP4
Identifiers
Aliases	MAP4, microtubule associated protein 4
External IDs	OMIM: 157132; MGI: 97178; GeneCards: MAP4
Gene location (Human) Chr. Chromosome 3 (human)[1] Band 3p21.31 Start 47,850,690 bp[1] End 48,089,272 bp[1]
Gene location (Mouse) Chr. Chromosome 9 (mouse)[2] Band 9 F2|9 59.83 cM Start 109,931,460 bp[2] End 110,083,955 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in dorsal motor nucleus of vagus nerve internal globus pallidus inferior olivary nucleus endothelial cell optic nerve tendon of biceps brachii C1 segment inferior ganglion of vagus nerve ventral tegmental area subthalamic nucleus Top expressed in neural layer of retina blastocyst muscle of thigh dentate gyrus of hippocampal formation granule cell morula cerebellar cortex mammillary body dorsomedial hypothalamic nucleus superior frontal gyrus sciatic nerve More reference expression data BioGPS n/a
Gene ontology Molecular function microtubule binding structural molecule activity tubulin binding protein binding RNA binding Cellular component microtubule associated complex neuron projection axoneme mitotic spindle microtubule extracellular exosome cytoskeleton cytoplasm postsynaptic density cytosol plasma membrane microtubule cytoskeleton axon Biological process mitotic spindle organization microtubule sliding cell division negative regulation of non-motile cilium assembly establishment of spindle orientation neuron projection development microtubule cytoskeleton organization Sources:Amigo / QuickGO
Orthologs Databases NCBI: entry; OMA: entry Species Human Mouse Entrez 4134 17758 Ensembl ENSG00000047849 ENSMUSG00000032479 UniProt P27816 P27546 RefSeq (mRNA) NM_001134364 NM_001134365 NM_002375 NM_030884 NM_030885 NM_001205330 NM_001205331 NM_001205332 NM_008633 NM_001311163 NM_001311164 RefSeq (protein) NP_001127836 NP_002366 NP_112147 NP_001192259 NP_001192260 NP_001192261 NP_001298092 NP_001298093 NP_032659 Location (UCSC) Chr 3: 47.85 – 48.09 Mb Chr 9: 109.93 – 110.08 Mb PubMed search [3] [4]
Wikidata
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Microtubule-associated protein 4 is a protein that in humans is encoded by the MAP4 gene.^[5]

The protein encoded by this gene is a major non-neuronal microtubule-associated protein. This protein contains a domain similar to the microtubule-binding domains of neuronal microtubule-associated protein (MAP2) and microtubule-associated protein tau (MAPT/TAU). This protein promotes microtubule assembly, and has been shown to counteract destabilization of interphase microtubule catastrophe promotion. Cyclin B was found to interact with this protein, which targets cell division cycle 2 (CDC2) kinase to microtubules. The phosphorylation of this protein affects microtubule properties and cell cycle progression. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed, the full-length nature of three of which are supported.^[6] uMAP4, the ubiquitous isoform of MAP4, functions in the architecture and positioning of the mitotic spindle in human cells.^[7] oMAP4 is predominantly expressed in brain and muscle and has been shown to organise microtubules into antiparallel bundles.^[8] mMAP4 is a muscle-specific isoform.^[8][9]

References

1. GRCh38: Ensembl release 89: ENSG00000047849 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000032479 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Chapin SJ, Bulinski JC (July 1991). "Non-neuronal 210 x 10(3) Mr microtubule-associated protein (MAP4) contains a domain homologous to the microtubule-binding domains of neuronal MAP2 and tau". Journal of Cell Science. 98. ( Pt 1): 27–36. doi:10.1242/jcs.98.1.27. PMID 1905296.
6. "Entrez Gene: MAP4 microtubule-associated protein 4".
7. Samora CP, Mogessie B, Conway L, Ross JL, Straube A, McAinsh AD (August 2011). "MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis". Nature Cell Biology. 13 (9): 1040–1050. doi:10.1038/ncb2297. PMID 21822276. S2CID 8869880.
8. Mogessie B, Roth D, Rahil Z, Straube A (April 2015). "A novel isoform of MAP4 organises the paraxial microtubule array required for muscle cell differentiation". eLife. 4 e05697. doi:10.7554/eLife.05697. PMC 4423121. PMID 25898002.
9. Casey LM, Lyon HD, Olmsted JB (April 2003). "Muscle-specific microtubule-associated protein 4 is expressed early in myogenesis and is not sufficient to induce microtubule reorganization". Cell Motility and the Cytoskeleton. 54 (4): 317–336. doi:10.1002/cm.10105. PMID 12601693.

Further reading

• Chapin SJ, Bulinski JC (1993). "Microtubule stabilization by assembly-promoting microtubule-associated proteins: a repeat performance". Cell Motility and the Cytoskeleton. 23 (4): 236–243. doi:10.1002/cm.970230403. PMID 1477887.
• West RR, Tenbarge KM, Olmsted JB (1991). "A model for microtubule-associated protein 4 structure. Domains defined by comparisons of human, mouse, and bovine sequences". The Journal of Biological Chemistry. 266 (32): 21886–21896. doi:10.1016/S0021-9258(18)54720-7. PMID 1718985.
• Chapin SJ, Lue CM, Yu MT, Bulinski JC (1995). "Differential expression of alternatively spliced forms of MAP4: a repertoire of structurally different microtubule-binding domains". Biochemistry. 34 (7): 2289–2301. doi:10.1021/bi00007a025. PMID 7857940.
• Ookata K, Hisanaga S, Bulinski JC, Murofushi H, Aizawa H, Itoh TJ, et al. (March 1995). "Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubules and is a potential regulator of M-phase microtubule dynamics". The Journal of Cell Biology. 128 (5): 849–862. doi:10.1083/jcb.128.5.849. PMC 2120387. PMID 7876309.
• Chapin SJ, Bulinski JC (1994). "Cellular microtubules heterogeneous in their content of microtubule-associated protein 4 (MAP4)". Cell Motility and the Cytoskeleton. 27 (2): 133–149. doi:10.1002/cm.970270205. PMID 7909279.
• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (April 1996). "A "double adaptor" method for improved shotgun library construction". Analytical Biochemistry. 236 (1): 107–113. doi:10.1006/abio.1996.0138. PMID 8619474.
• Illenberger S, Drewes G, Trinczek B, Biernat J, Meyer HE, Olmsted JB, et al. (May 1996). "Phosphorylation of microtubule-associated proteins MAP2 and MAP4 by the protein kinase p110mark. Phosphorylation sites and regulation of microtubule dynamics". The Journal of Biological Chemistry. 271 (18): 10834–10843. doi:10.1074/jbc.271.18.10834. PMID 8631898.
• Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, et al. (April 1997). "Large-scale concatenation cDNA sequencing". Genome Research. 7 (4): 353–358. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
• Bulinski JC, McGraw TE, Gruber D, Nguyen HL, Sheetz MP (December 1997). "Overexpression of MAP4 inhibits organelle motility and trafficking in vivo". Journal of Cell Science. 110. 110 ( Pt 24) (24): 3055–3064. doi:10.1242/jcs.110.24.3055. PMID 9365275.
• Ookata K, Hisanaga S, Sugita M, Okuyama A, Murofushi H, Kitazawa H, et al. (December 1997). "MAP4 is the in vivo substrate for CDC2 kinase in HeLa cells: identification of an M-phase specific and a cell cycle-independent phosphorylation site in MAP4". Biochemistry. 36 (50): 15873–15883. doi:10.1021/bi971251w. PMID 9398320.
• Nguyen HL, Gruber D, Bulinski JC (1999). "Microtubule-associated protein 4 (MAP4) regulates assembly, protomer-polymer partitioning and synthesis of tubulin in cultured cells". Journal of Cell Science. 112. ( Pt 12) (12): 1813–1824. doi:10.1242/jcs.112.12.1813. PMID 10341201.
• Ebneth A, Drewes G, Mandelkow EM, Mandelkow E (2000). "Phosphorylation of MAP2c and MAP4 by MARK kinases leads to the destabilization of microtubules in cells". Cell Motility and the Cytoskeleton. 44 (3): 209–224. doi:10.1002/(SICI)1097-0169(199911)44:3<209::AID-CM6>3.0.CO;2-4. PMID 10542369.
• Kitazawa H, Iida J, Uchida A, Haino-Fukushima K, Itoh TJ, Hotani H, et al. (February 2000). "Ser787 in the proline-rich region of human MAP4 is a critical phosphorylation site that reduces its activity to promote tubulin polymerization". Cell Structure and Function. 25 (1): 33–39. doi:10.1247/csf.25.33. PMID 10791892.
• Chang W, Gruber D, Chari S, Kitazawa H, Hamazumi Y, Hisanaga S, et al. (August 2001). "Phosphorylation of MAP4 affects microtubule properties and cell cycle progression". Journal of Cell Science. 114 (Pt 15): 2879–2887. doi:10.1242/jcs.114.15.2879. PMID 11683421.
• Iida J, Itoh TJ, Hotani H, Nishiyama K, Murofushi H, Bulinski JC, et al. (June 2002). "The projection domain of MAP4 suppresses the microtubule-bundling activity of the microtubule-binding domain". Journal of Molecular Biology. 320 (1): 97–106. doi:10.1016/S0022-2836(02)00402-3. PMID 12079337.
• Holmfeldt P, Brattsand G, Gullberg M (2002). "MAP4 counteracts microtubule catastrophe promotion but not tubulin-sequestering activity in intact cells". Current Biology. 12 (12): 1034–1039. Bibcode:2002CBio...12.1034H. doi:10.1016/S0960-9822(02)00897-7. PMID 12123579. S2CID 8816838.
• Tokuraku K, Matsushima K, Matui T, Nakagawa H, Katsuki M, Majima R, et al. (August 2003). "The number of repeat sequences in microtubule-associated protein 4 affects the microtubule surface properties". The Journal of Biological Chemistry. 278 (32): 29609–29618. doi:10.1074/jbc.M302186200. PMID 12773533.
• Mangan ME, Olmsted J (1996). "The gene for microtubule-associated protein 4 (Mtap4) maps to the distal region of mouse chromosome 9". Mammalian Genome. 7 (12): 918–919. doi:10.1007/s003359900270. PMID 8995766. S2CID 43334937.