Asyterot-Karnaim (bahasa Ibrani: עַשְׁתְּרֹת קַרְנַיִם ʿAštərōṯ Qarnayīm), juga disebut sebagai Asytarot-Karnaim, adalah sebuah kota di tanah Bashan di timur Sungai Yordan.
Asyterot-Karnaim disebutkan dalam Kitab Kejadian (Kejadian 14:5, dan dalam Kitab Yosua (Yosua 12:4) dimana kota tersebut disebut sebagai "Asytarot". Karnaim juga disebutkan oleh nabi Amos (Kitab Amos 6:13).
Karnaeim. Astaroth Karnaeim. There is now a large village of Arabia (in a corner of the Batanea) which is called Karnaia beyond the (river) Jordan. There according to tradition the house of Job is pointed out.
The two villages are best located at Tell 'ashtarah and Sheih Sa'ad. The former is a large tell suitable for the Old Testament Ashtaroth (cf. K. 12:11). Perhaps the latter succeeded as chief administrative city of the district of Karnaeim (cf. K. 112:3). However in the Bible, Astaroth is merely identifying the site of a battle which took place near the city. If so, then Karnaeim added to the name gives the district in which the battle took place (cf. Biblical Archaeologist Dec. 1962, p.109). Eusebius seems to look for two sites.