Kompleks prepoznavanja mesta početka, podjedinica 2

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Kompleks prepoznavanja mesta početka, podjedinica 2
Identifikatori
Simboli	ORC2; ORC2L
Vanjski ID	OMIM: 601182 MGI: 1328306 HomoloGene: 4512 GeneCards: ORC2 Gene
Ontologija gena Molekulska funkcija • vezivanje za mesto početka DNK replikacije • proteinsko vezivanje Ćelijska komponenta • heterohromatin • Kompleks prepoznavanja mesta početka • kondenzovani hromozom unutrašnjeg kinetohora Biološki proces • kontrolna tačka ćelijskog ciklusa • G1/S tranzicija mitotičkog ćelijskog ciklusa • S faza mitotičkog ćelijskog ciklusa
Pregled RNK izražavanja
podaci
Ortolozi
Vrsta	Čovek	Miš
Entrez	4999	18393
Ensembl	ENSG00000115942	ENSMUSG00000026037
UniProt	Q13416	Q60862
Ref. Sekv. (iRNK)	NM_006190	NM_001025378
Ref. Sekv. (protein)	NP_006181	NP_001020549
Lokacija (UCSC)	Chr 2: 201.77 - 201.83 Mb	Chr 1: 58.46 - 58.51 Mb
PubMed pretraga	[1]	[2]

Origin recognition complex subunit 2

Identifikatori
Simbol	ORC2
Pfam	PF04084
InterPro	IPR007220
Dostupne PDB strukture: Pfam structures PDB RCSB PDB; PDBe PDBsum strukturni pregled

Kompleks prepoznavanja mesta početka, podjedinica 2 je protein koji je kod ljudi kodiran ORC2 (ORC2L) genom.^[1][2]

Kompleks prepoznavanja mesta početka (ORC) je visoko konzervirani proteinski kompleks sa šest podjedinica koji je esencijalan za inicijaciju replikacije DNK kod eukariotskih ćelija. Istraživanja na kvascu su pokazala da se ORC specifično vezuje za mesto početka replikacije i da služi kao platforma za konstrukciju prereplikacionog kompleksa, koji sadrži dodatne inicijacione faktore kao što su Cdc6 i Mcm proteini. Protein kodiran ovim genom je podjedinica kompleksa prepoznavanja mesta početka. Ovaj protein formira sržni kompleks sa ORC3, ORC4, i ORC5. On takođe ostvaruje interakcije sa CDC45L i MCM10, koji su važni za DNK replikaciju. Za ovaj protein je pokazano da se specifično asocira sa mestom početka replikacije Epstein-Barr virusa u ljudskim ćelijama, i smatra se da je neophodan za DNK replikacju iz mesta početka virusa.^[2]

Interakcije

ORC2 formira interakcije sa ORC1,^[3][4][5][6] replikacionim proteinom A1,^[3] ORC4,^[3][5][7][8] ORC5,^[3][5][7][9] ORC3,^{[3][5][7][10][11]} MCM4,^[3] CDC6,^[3][12] MCM5,^[3] MCM6,^[3] MCM7,^[3] MCM10,^[13] DBF4,^[3] MCM2^[3] i ORC6.^[3][5]

Reference

1. Takahara K, Bong M, Brevard R, Eddy RL, Haley LL, Sait SJ, Shows TB, Hoffman GG, Greenspan DS (October 1996). „Mouse and human homologues of the yeast origin of replication recognition complex subunit ORC2 and chromosomal localization of the cognate human gene ORC2L”. Genomics 31 (1): 119–122. DOI:10.1006/geno.1996.0018. PMID 8808289. 
2. „Entrez Gene: ORC2L origin recognition complex, subunit 2-like (yeast)”. 
3. Kneissl, Margot; Pütter Vera, Szalay Aladar A, Grummt Friedrich (March 2003). „Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells”. J. Mol. Biol. (England) 327 (1): 111–128. DOI:10.1016/S0022-2836(03)00079-2. ISSN 0022-2836. PMID 12614612. 
4. Fujita, Masatoshi; Ishimi Yukio, Nakamura Hiromu, Kiyono Tohru, Tsurumi Tatsuya (March 2002). „Nuclear organization of DNA replication initiation proteins in mammalian cells”. J. Biol. Chem. (United States) 277 (12): 10354–10361. DOI:10.1074/jbc.M111398200. ISSN 0021-9258. PMID 11779870. 
5. Vashee, S; Simancek P, Challberg M D, Kelly T J (July 2001). „Assembly of the human origin recognition complex”. J. Biol. Chem. (United States) 276 (28): 26666–26673. DOI:10.1074/jbc.M102493200. ISSN 0021-9258. PMID 11323433. 
6. Méndez, Juan; Zou-Yang X Helena, Kim So-Young, Hidaka Masumi, Tansey William P, Stillman Bruce (March 2002). „Human origin recognition complex large subunit is degraded by ubiquitin-mediated proteolysis after initiation of DNA replication”. Mol. Cell (United States) 9 (3): 481–491. DOI:10.1016/S1097-2765(02)00467-7. ISSN 1097-2765. PMID 11931757. 
7. Dhar, S K; Delmolino L, Dutta A (August 2001). „Architecture of the human origin recognition complex”. J. Biol. Chem. (United States) 276 (31): 29067–29071. DOI:10.1074/jbc.M103078200. ISSN 0021-9258. PMID 11395502. 
8. Quintana, D G; Hou Zh, Thome K C, Hendricks M, Saha P, Dutta A (November 1997). „Identification of HsORC4, a member of the human origin of replication recognition complex”. J. Biol. Chem. (United States) 272 (45): 28247–28251. DOI:10.1074/jbc.272.45.28247. ISSN 0021-9258. PMID 9353276. 
9. Quintana, D G; Thome K C, Hou Z H, Ligon A H, Morton C C, Dutta A (October 1998). „ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases”. J. Biol. Chem. (United States) 273 (42): 27137–27145. DOI:10.1074/jbc.273.42.27137. ISSN 0021-9258. PMID 9765232. 
10. Matsuoka, Shuhei; Ballif Bryan A, Smogorzewska Agata, McDonald E Robert, Hurov Kristen E, Luo Ji, Bakalarski Corey E, Zhao Zhenming, Solimini Nicole, Lerenthal Yaniv, Shiloh Yosef, Gygi Steven P, Elledge Stephen J (May 2007). „ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage”. Science (United States) 316 (5828): 1160–1166. DOI:10.1126/science.1140321. PMID 17525332. 
11. Pinto, S; Quintana D G, Smith P, Mihalek R M, Hou Z H, Boynton S, Jones C J, Hendricks M, Velinzon K, Wohlschlegel J A, Austin R J, Lane W S, Tully T, Dutta A (May 1999). „latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant”. Neuron (United States) 23 (1): 45–54. DOI:10.1016/S0896-6273(00)80752-7. ISSN 0896-6273. PMID 10402192. 
12. Méndez, J; Stillman B (November 2000). „Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis”. Mol. Cell. Biol. (United States) 20 (22): 8602–8612. DOI:10.1128/MCB.20.22.8602-8612.2000. ISSN 0270-7306. PMC 102165. PMID 11046155. 
13. Izumi, M; Yanagi K, Mizuno T, Yokoi M, Kawasaki Y, Moon K Y, Hurwitz J, Yatagai F, Hanaoka F (December 2000). „The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase”. Nucleic Acids Res. (ENGLAND) 28 (23): 4769–4777. DOI:10.1093/nar/28.23.4769. PMC 115166. PMID 11095689. 

Literatura

• Gavin KA, Hidaka M, Stillman B (1996). „Conserved initiator proteins in eukaryotes”. Science 270 (5242): 1667–1671. DOI:10.1126/science.270.5242.1667. PMID 7502077. 
• Quintana DG, Hou Zh, Thome KC i dr.. (1997). „Identification of HsORC4, a member of the human origin of replication recognition complex”. J. Biol. Chem. 272 (45): 28247–28251. DOI:10.1074/jbc.272.45.28247. PMID 9353276. 
• Saha P, Thome KC, Yamaguchi R i dr.. (1998). „The human homolog of Saccharomyces cerevisiae CDC45”. J. Biol. Chem. 273 (29): 18205–18209. DOI:10.1074/jbc.273.29.18205. PMID 9660782. 
• Ritzi M, Baack M, Musahl C i dr.. (1998). „Human minichromosome maintenance proteins and human origin recognition complex 2 protein on chromatin”. J. Biol. Chem. 273 (38): 24543–24549. DOI:10.1074/jbc.273.38.24543. PMID 9733749. 
• Quintana DG, Thome KC, Hou ZH i dr.. (1998). „ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases”. J. Biol. Chem. 273 (42): 27137–27145. DOI:10.1074/jbc.273.42.27137. PMID 9765232. 
• Pinto S, Quintana DG, Smith P i dr.. (1999). „latheo encodes a subunit of the origin recognition complex and disrupts neuronal proliferation and adult olfactory memory when mutant”. Neuron 23 (1): 45–54. DOI:10.1016/S0896-6273(00)80752-7. PMID 10402192. 
• Jiang W, McDonald D, Hope TJ, Hunter T (1999). „Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication”. EMBO J. 18 (20): 5703–5713. DOI:10.1093/emboj/18.20.5703. PMC 1171637. PMID 10523313. 
• Natale DA, Li CJ, Sun WH, DePamphilis ML (2000). „Selective instability of Orc1 protein accounts for the absence of functional origin recognition complexes during the M-G(1) transition in mammals”. EMBO J. 19 (11): 2728–2738. DOI:10.1093/emboj/19.11.2728. PMC 212765. PMID 10835370. 
• Thome KC, Dhar SK, Quintana DG i dr.. (2001). „Subsets of human origin recognition complex (ORC) subunits are expressed in non-proliferating cells and associate with non-ORC proteins”. J. Biol. Chem. 275 (45): 35233–35241. DOI:10.1074/jbc.M005765200. PMID 10954718. 
• Izumi M, Yanagi K, Mizuno T i dr.. (2001). „The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase”. Nucleic Acids Res. 28 (23): 4769–4777. DOI:10.1093/nar/28.23.4769. PMC 115166. PMID 11095689. 
• Vashee S, Simancek P, Challberg MD, Kelly TJ (2001). „Assembly of the human origin recognition complex”. J. Biol. Chem. 276 (28): 26666–26673. DOI:10.1074/jbc.M102493200. PMID 11323433. 
• Dhar SK, Delmolino L, Dutta A (2001). „Architecture of the human origin recognition complex”. J. Biol. Chem. 276 (31): 29067–29071. DOI:10.1074/jbc.M103078200. PMID 11395502. 
• Dhar SK, Yoshida K, Machida Y i dr.. (2001). „Replication from oriP of Epstein-Barr virus requires human ORC and is inhibited by geminin”. Cell 106 (3): 287–296. DOI:10.1016/S0092-8674(01)00458-5. PMID 11509178. 
• Izumi M, Yatagai F, Hanaoka F (2002). „Cell cycle-dependent proteolysis and phosphorylation of human Mcm10”. J. Biol. Chem. 276 (51): 48526–31. DOI:10.1074/jbc.M107190200. PMID 11602595. 
• Fujita M, Ishimi Y, Nakamura H i dr.. (2002). „Nuclear organization of DNA replication initiation proteins in mammalian cells”. J. Biol. Chem. 277 (12): 10354–10361. DOI:10.1074/jbc.M111398200. PMID 11779870. 
• Schaarschmidt D, Ladenburger EM, Keller C, Knippers R (2002). „Human Mcm proteins at a replication origin during the G1 to S phase transition”. Nucleic Acids Res. 30 (19): 4176–4185. DOI:10.1093/nar/gkf532. PMC 140533. PMID 12364596. 
• Matheos D, Ruiz MT, Price GB, Zannis-Hadjopoulos M (2002). „Ku antigen, an origin-specific binding protein that associates with replication proteins, is required for mammalian DNA replication”. Biochim. Biophys. Acta 1578 (1–3): 59–72. DOI:10.1016/S0167-4781(02)00497-9. PMID 12393188. 
• Strausberg RL, Feingold EA, Grouse LH i dr.. (2003). „Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences”. Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–16903. DOI:10.1073/pnas.242603899. PMC 139241. PMID 12477932. 
• Kneissl M, Pütter V, Szalay AA, Grummt F (2003). „Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells”. J. Mol. Biol. 327 (1): 111–128. DOI:10.1016/S0022-2836(03)00079-2. PMID 12614612.