Podjedinica A ribonukleaze H2 , takođe poznata kao podjedinica A RNaze H2 , je enzim koji je kod ljudi kodiran RNASEH2A genom .[ 1]
Protein kodiran ovim genom je komponenta heterotrimerne ribonukleaze H tipa II (RNAseH2). RNAseH2 je glavni izvor ribonukleazne H aktivnosti u ćelijama sisara. Ona endonukleolitički preseca ribonukleotide . Smatra se da uklanja Okazakijev fragment RNK prajmera tokom sinteze zaostajućeg lanca DNK i da iseca pojedinačne ribonukleotide iz DNK-DNK dupleksa.[ 1]
Mutacije ovog gena uzrokuju Aicardi–Goutières sindrom (AGS), autozomalno recesivni neurološki poremećaj karakterisan progresivnom mikrocefalijom i psihomotornom retardacijom, intrakranijalnom kalcifikacijom, povišenim nivoima interferona alfa i belih krvni zrnca u cerebrospinalnom fluidu .[ 1]
Crow YJ, Leitch A, Hayward BE, et al. (2006). „Mutations in genes encoding ribonuclease H2 subunits cause Aicardi-Goutières syndrome and mimic congenital viral brain infection.”. Nat. Genet. 38 (8): 910–6. DOI :10.1038/ng1842 . PMID 16845400 . Chon H, Vassilev A, DePamphilis ML, et al. (2009). „Contributions of the two accessory subunits, RNASEH2B and RNASEH2C, to the activity and properties of the human RNase H2 complex” . Nucleic Acids Res. 37 (1): 96–110. DOI :10.1093/nar/gkn913 . PMC 2615623 . PMID 19015152 . Flanagan JM, Funes JM, Henderson S, et al. (2009). „Genomics screen in transformed stem cells reveals RNASEH2A, PPAP2C, and ADARB1 as putative anticancer drug targets”. Mol. Cancer Ther. 8 (1): 249–60. DOI :10.1158/1535-7163.MCT-08-0636 . PMID 19139135 . Gerhard DS, Wagner L, Feingold EA, et al. (2004). „The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)” . Genome Res. 14 (10B): 2121–7. DOI :10.1101/gr.2596504 . PMC 528928 . PMID 15489334 . Bonaldo MF, Lennon G, Soares MB (1996). „Normalization and subtraction: two approaches to facilitate gene discovery”. Genome Res. 6 (9): 791–806. DOI :10.1101/gr.6.9.791 . PMID 8889548 . Rice G, Patrick T, Parmar R, et al. (2007). „Clinical and Molecular Phenotype of Aicardi-Goutières Syndrome” . Am. J. Hum. Genet. 81 (4): 713–25. DOI :10.1086/521373 . PMC 2227922 . PMID 17846997 . Frank P, Braunshofer-Reiter C, Wintersberger U, et al. (1998). „Cloning of the cDNA encoding the large subunit of human RNase HI, a homologue of the prokaryotic RNase HII” . Proc. Natl. Acad. Sci. U.S.A. 95 (22): 12872–7. DOI :10.1073/pnas.95.22.12872 . PMC 23637 . PMID 9789007 . Strausberg RL, Feingold EA, Grouse LH, et al. (2002). „Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences” . Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. DOI :10.1073/pnas.242603899 . PMC 139241 . PMID 12477932 . Ganesh SK, Zakai NA, van Rooij FJ, et al. (2009). „Multiple loci influence erythrocyte phenotypes in the CHARGE Consortium” . Nat. Genet. 41 (11): 1191–8. DOI :10.1038/ng.466 . PMC 2778265 . PMID 19862010 .
3.1.1 : Hidrolaze karboksilnih estaraHolinesteraza (
Acetilholinesteraza ,
Butirilholinesteraza )
• Pektinesteraza • 6-fosfoglukonolaktonaza • PAF acetilhidrolaza
Lipaza (Zavisna od žučnih soli , Gastrična /Lingvalna , Pankreasna , Lizozomalna , Hormon-sensitivna , Endotelna , Hepatička , Lipoproteinska , Monoacilglicerolna , Diacilglicerolna )
Fosfolipaza (
A1 ,
A2 ,
B )
3.1.2 : Tioesteraza 3.1.3 : Fosfataza 3.1.4 : Fosfodiesteraza 3.1.6 : Sulfataza Nukleaza (obuhvatadeoksiribonukleazu iribonukleazu )
Separacija
Replikacija
DNK polimeraza III holoenzim (
dnaC ,
dnaE ,
dnaH ,
dnaN ,
dnaQ ,
dnaT ,
dnaX ,
holA ,
holB ,
holC ,
holD ,
holE )
• Replizom • DNK ligaza • DNK stega • Topoizomeraza (
DNK giraza )
Prokariotska DNK polimeraza :
DNK polimeraza I (
Klenov fragment )
Oba
Terminacija B bsyn : dnk (repl , cycl , reco , repr ) • tscr (fact , tcrg , nucl , rnat , rept , ptts ) • tltn (risu , pttl , nexn ) • dnab , rnab /runp • stru (domn , 1° , 2° , 3° , 4° )