Limfotoksin beta receptor

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Limfotoksin beta receptor (TNFR superfamilija, član 3)
Kristalografska struktura peptida sa 24 ostatka iz limfotoksin-B receptora vezanog za TRAF3.[1]
Dostupne strukture
1RF3
Identifikatori
Simboli	LTBR; CD18; D12S370; LT-BETA-R; TNF-R-III; TNFCR; TNFR-RP; TNFR2-RP; TNFRSF3
Vanjski ID	OMIM: 600979 MGI: 104875 HomoloGene: 1753 GeneCards: LTBR Gene
Ontologija gena Molekularna funkcija • aktivnost transmembranskog receptora • proteinsko vezivanje Celularna komponenta • membrana • integralno sa membranom Biološki proces • apoptoza • imunski respons • prenos signala • positivna regulacija I-kapaB kinaza/NF-kapaB kaskade
Pregled RNK izražavanja
podaci
Ortolozi
Vrsta	Čovek	Miš
Entrez	4055	17000
Ensembl	ENSG00000111321	n/a
UniProt	P36941	n/a
RefSeq (mRNA)	NM_002342	XM_982227
RefSeq (protein)	NP_002333	XP_987321
Lokacija (UCSC)	Chr 12: 6.36 - 6.37 Mb	n/a
PubMed pretraga	[1]	[2]

Limfotoksin beta receptor je receptor za limfotoksin koji je kod ljudi kodiran LTBR genom.^[2][3][4]

Funkcija

Protein kodiran ovim genom je član TNF familije receptora. On je izražen na površini većine ćelijskih tipova, uključujući ćelije epitelnog i mijeloidnog porekla, ali ne na T i B limfocitima. Ovaj protein specifično vezuje limfotoksin membransku formu (kompleks limfotoksina-alfa i limftoksina-beta se formira). Limftoksin-beta i njegov ligand učestvuju u razvoju i organizaciji limfoidnog tkiva. Aktivacija ovog proteina može da podstakne apoptozu.^[2]

Ne samp da LTBR pomaže u podsticanju apoptoze, on može da dovede do oslobađanja citokina interleukin 8. Prekomerno LTBR izražavanje u HEK293 ćelima povećava IL-8 promotorsku aktivnost, i dovodi do IL-8 oslobađanja. LTBR je takođe esencijalan za razvoj i organizaciju sekundarnih limfoidnih organa i oslobađanje drugih hemokina.^[5]

Struktura

Ramačandranov dijagram pokazuje da je 64.6% ostataka u poželjnom regionu.^[6] Ova structura je ustanovljena putem difrakcije X-zraka. Njena rezolucija je 3.50 Å. Alfa i beta uglovi su 90 stepeni, dok je gama ugao 120 stepeni.

Interakcije

Za limfotoksin beta receptor je bilo pokazano da interaguje sa Dijablo homologom^[7] i TRAF3.^[8][9][10]

Reference

1. ’PDB 1RF3; Li C, Norris PS, Ni CZ, Havert ML, Chiong EM, Tran BR, Cabezas E, Reed JC, Satterthwait AC, Ware CF, Ely KR (December 2003). „Structurally distinct recognition motifs in lymphotoxin-beta receptor and CD40 for tumor necrosis factor receptor-associated factor (TRAF)-mediated signaling”. J. Biol. Chem. 278 (50): 50523–9. DOI:10.1074/jbc.M309381200. PMID 14517219. 
2. „Entrez Gene: LTBR lymphotoxin beta receptor (TNFR superfamily, member 3)”. 
3. Baens M, Chaffanet M, Cassiman JJ, van den Berghe H, Marynen P (April 1993). „Construction and evaluation of a hncDNA library of human 12p transcribed sequences derived from a somatic cell hybrid”. Genomics 16 (1): 214–8. DOI:10.1006/geno.1993.1161. PMID 8486360. 
4. Crowe PD, VanArsdale TL, Walter BN, Ware CF, Hession C, Ehrenfels B, Browning JL, Din WS, Goodwin RG, Smith CA (April 1994). „A lymphotoxin-beta-specific receptor”. Science (journal) 264 (5159): 707–10. DOI:10.1126/science.8171323. PMID 8171323. 
5. Chang YH, Hsieh SL, Chen MC, Lin WW (August 2002). „Lymphotoxin beta receptor induces interleukin 8 gene expression via NF-kappaB and AP-1 activation”. Exp. Cell Res. 278 (2): 166–74. DOI:10.1006/excr.2002.5573. PMID 12169272. 
6. „MolProbity Ramachandran analysis”. Arhivirano iz originala na datum 2012-10-12. 
7. Kuai, Jun; Nickbarg Elliott, Wooters Joe, Qiu Yongchang, Wang Jack, Lin Lih-Ling (April 2003). „Endogenous association of TRAF2, TRAF3, cIAP1, and Smac with lymphotoxin beta receptor reveals a novel mechanism of apoptosis”. J. Biol. Chem. (United States) 278 (16): 14363–9. DOI:10.1074/jbc.M208672200. ISSN 0021-9258. PMID 12571250. 
8. VanArsdale, T L; VanArsdale S L, Force W R, Walter B N, Mosialos G, Kieff E, Reed J C, Ware C F (March 1997). „Lymphotoxin-beta receptor signaling complex: role of tumor necrosis factor receptor-associated factor 3 recruitment in cell death and activation of nuclear factor kappaB”. Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 94 (6): 2460–5. DOI:10.1073/pnas.94.6.2460. ISSN 0027-8424. PMC 20110. PMID 9122217. 
9. Wu, M Y; Wang P Y, Han S H, Hsieh S L (April 1999). „The cytoplasmic domain of the lymphotoxin-beta receptor mediates cell death in HeLa cells”. J. Biol. Chem. (UNITED STATES) 274 (17): 11868–73. DOI:10.1074/jbc.274.17.11868. ISSN 0021-9258. PMID 10207006. 
10. Marsters, S A; Ayres T M, Skubatch M, Gray C L, Rothe M, Ashkenazi A (May 1997). „Herpesvirus entry mediator, a member of the tumor necrosis factor receptor (TNFR) family, interacts with members of the TNFR-associated factor family and activates the transcription factors NF-kappaB and AP-1”. J. Biol. Chem. (UNITED STATES) 272 (22): 14029–32. DOI:10.1074/jbc.272.22.14029. ISSN 0021-9258. PMID 9162022. 

Literatura

• Elewaut D, Ware CF (2007). „The unconventional role of LT alpha beta in T cell differentiation.”. Trends Immunol. 28 (4): 169–75. DOI:10.1016/j.it.2007.02.005. PMID 17336158. 
• Browning JL, Ngam-ek A, Lawton P, et al. (1993). „Lymphotoxin beta, a novel member of the TNF family that forms a heteromeric complex with lymphotoxin on the cell surface.”. Cell 72 (6): 847–56. DOI:10.1016/0092-8674(93)90574-A. PMID 7916655. 
• Crowe PD, VanArsdale TL, Walter BN, et al. (1994). „A lymphotoxin-beta-specific receptor.”. Science 264 (5159): 707–10. DOI:10.1126/science.8171323. PMID 8171323. 
• Baens M, Chaffanet M, Cassiman JJ, et al. (1993). „Construction and evaluation of a hncDNA library of human 12p transcribed sequences derived from a somatic cell hybrid.”. Genomics 16 (1): 214–8. DOI:10.1006/geno.1993.1161. PMID 8486360. 
• Baens M, Aerssens J, van Zand K, et al. (1996). „Isolation and regional assignment of human chromosome 12p cDNAs.”. Genomics 29 (1): 44–52. DOI:10.1006/geno.1995.1213. PMID 8530100. 
• Wang X, Bornslaeger EA, Haub O, et al. (1996). „A candidate gene for the amnionless gastrulation stage mouse mutation encodes a TRAF-related protein.”. Dev. Biol. 177 (1): 274–90. DOI:10.1006/dbio.1996.0162. PMID 8660894. 
• Nakano H, Oshima H, Chung W, et al. (1996). „TRAF5, an activator of NF-kappaB and putative signal transducer for the lymphotoxin-beta receptor.”. J. Biol. Chem. 271 (25): 14661–4. DOI:10.1074/jbc.271.25.14661. PMID 8663299. 
• Matsumoto M, Hsieh TY, Zhu N, et al. (1997). „Hepatitis C virus core protein interacts with the cytoplasmic tail of lymphotoxin-beta receptor.”. J. Virol. 71 (2): 1301–9. PMC 191185. PMID 8995654. 
• VanArsdale TL, VanArsdale SL, Force WR, et al. (1997). „Lymphotoxin-beta receptor signaling complex: role of tumor necrosis factor receptor-associated factor 3 recruitment in cell death and activation of nuclear factor kappaB.”. Proc. Natl. Acad. Sci. U.S.A. 94 (6): 2460–5. DOI:10.1073/pnas.94.6.2460. PMC 20110. PMID 9122217. 
• Wu MY, Hsu TL, Lin WW, et al. (1997). „Serine/threonine kinase activity associated with the cytoplasmic domain of the lymphotoxin-beta receptor in HepG2 cells.”. J. Biol. Chem. 272 (27): 17154–9. DOI:10.1074/jbc.272.27.17154. PMID 9202035. 
• Chen CM, You LR, Hwang LH, Lee YH (1997). „Direct interaction of hepatitis C virus core protein with the cellular lymphotoxin-beta receptor modulates the signal pathway of the lymphotoxin-beta receptor.”. J. Virol. 71 (12): 9417–26. PMC 230246. PMID 9371602. 
• Mizushima S, Fujita M, Ishida T, et al. (1998). „Cloning and characterization of a cDNA encoding the human homolog of tumor necrosis factor receptor-associated factor 5 (TRAF5).”. Gene 207 (2): 135–40. DOI:10.1016/S0378-1119(97)00616-1. PMID 9511754. 
• Krajewska M, Krajewski S, Zapata JM, et al. (1998). „TRAF-4 expression in epithelial progenitor cells. Analysis in normal adult, fetal, and tumor tissues.”. Am. J. Pathol. 152 (6): 1549–61. PMC 1858434. PMID 9626059. 
• Boussaud V, Soler P, Moreau J, et al. (1999). „Expression of three members of the TNF-R family of receptors (4-1BB, lymphotoxin-beta receptor, and Fas) in human lung.”. Eur. Respir. J. 12 (4): 926–31. DOI:10.1183/09031936.98.12040926. PMID 9817170. 
• Murphy M, Walter BN, Pike-Nobile L, et al. (1999). „Expression of the lymphotoxin beta receptor on follicular stromal cells in human lymphoid tissues.”. Cell Death Differ. 5 (6): 497–505. DOI:10.1038/sj.cdd.4400374. PMID 10200501. 
• Wu MY, Wang PY, Han SH, Hsieh SL (1999). „The cytoplasmic domain of the lymphotoxin-beta receptor mediates cell death in HeLa cells.”. J. Biol. Chem. 274 (17): 11868–73. DOI:10.1074/jbc.274.17.11868. PMID 10207006. 
• Yu KY, Kwon B, Ni J, et al. (1999). „A newly identified member of tumor necrosis factor receptor superfamily (TR6) suppresses LIGHT-mediated apoptosis.”. J. Biol. Chem. 274 (20): 13733–6. DOI:10.1074/jbc.274.20.13733. PMID 10318773. 
• Rooney IA, Butrovich KD, Glass AA, et al. (2000). „The lymphotoxin-beta receptor is necessary and sufficient for LIGHT-mediated apoptosis of tumor cells.”. J. Biol. Chem. 275 (19): 14307–15. DOI:10.1074/jbc.275.19.14307. PMID 10799510. 
• Langeggen H, Berge KE, Johnson E, Hetland G (2003). „Human umbilical vein endothelial cells express complement receptor 1 (CD35) and complement receptor 4 (CD11c/CD18) in vitro.”. Inflammation 26 (3): 103–10. DOI:10.1023/A:1015585530204. PMID 12083416. 
• Chang YH, Hsieh SL, Chen MC, Lin WW (2002). „Lymphotoxin beta receptor induces interleukin 8 gene expression via NF-kappaB and AP-1 activation.”. Exp. Cell Res. 278 (2): 166–74. DOI:10.1006/excr.2002.5573. PMID 12169272. 

Spoljašnje veze

• MeSH Lymphotoxin+beta+Receptor