Podjedinica IIIB transkripcijskog faktora , od 50 kDa (TFIIIB50), znana i kao b-srodni faktor 2 (BRF-2) je protein koji je kod ljudi kodiran genom BRF2 .[ 1] [ 2] [ 3]
Dužina polipeptidnog lanca je 419 aminokiselina , а molekulska težina 46.533 Da .[ 4]
10 20 30 40 50 MPGRGRCPDC GSTELVEDSH YSQSQLVCSD CGCVVTEGVL TTTFSDEGNL REVTYSRSTG ENEQVSRSQQ RGLRRVRDLC RVLQLPPTFE DTAVAYYQQA YRHSGIRAAR LQKKEVLVGC CVLITCRQHN WPLTMGAICT LLYADLDVFS STYMQIVKLL GLDVPSLCLA ELVKTYCSSF KLFQASPSVP AKYVEDKEKM LSRTMQLVEL ANETWLVTGR HPLPVITAAT FLAWQSLQPA DRLSCSLARF CKLANVDLPY PASSRLQELL AVLLRMAEQL AWLRVLRLDK RSVVKHIGDL LQHRQSLVRS AFRDGTAEVE TREKEPPGWG QGQGEGEVGN NSLGLPQGKR PASPALLLPP CMLKSPKRIC PVPPVSTVTG DENISDSEIE QYLRTPQEVR DFQRAQAARQ AATSVPNPP
Ovaj gen kodira jednu od više podjedinica kompleksa transkripcijskog faktora RNK-polimeraze III, potrebnih za transkripciju gena sa elementima promotora uzvodno od mjesta inicijacije. Produkt ovog gena, faktor sličan TFIIB , direktno se regrutira u TATA-kutiju promotora gena male jedarne RNK-polimeraze III, njegovom interakcijom sa TATA-vezanim proteinom .[ 3]
^ Yu Y, Zhang C, Zhou G, Wu S, Qu X, Wei H, Xing G, Dong C, Zhai Y, Wan J, Ouyang S, Li L, Zhang S, Zhou K, Zhang Y, Wu C, He F (Aug 2001). "Gene expression profiling in human fetal liver and identification of tissue- and developmental-stage-specific genes through compiled expression profiles and efficient cloning of full-length cDNAs" . Genome Res . 11 (8): 1392–403. doi :10.1101/gr.175501 . PMC 311073 PMID 11483580 . ^ Cabart P, Murphy S (Nov 2001). "BRFU, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with TATA-binding protein" . J Biol Chem . 276 (46): 43056–64. doi :10.1074/jbc.M108515200 PMID 11564744 . ^ a b "Entrez Gene: BRF2 BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like" .^ "UniProt, Q9HAW0" . Pristupljeno 14. 9. 2021 .
Sutcliffe JE, Cairns CA, McLees A, et al. (1999). "RNA polymerase III transcription factor IIIB is a target for repression by pocket proteins p107 and p130" . Mol. Cell. Biol . 19 (6): 4255–61. doi :10.1128/mcb.19.6.4255 . PMC 104385 PMID 10330166 . Schramm L, Pendergrast PS, Sun Y, Hernandez N (2000). "Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters" . Genes Dev . 14 (20): 2650–63. doi :10.1101/gad.836400 . PMC 316990 PMID 11040218 . Teichmann M, Wang Z, Roeder RG (2001). "A stable complex of a novel transcription factor IIB- related factor, human TFIIIB50, and associated proteins mediate selective transcription by RNA polymerase III of genes with upstream promoter elements" . Proc. Natl. Acad. Sci. U.S.A . 97 (26): 14200–5. doi :10.1073/pnas.97.26.14200 . PMC 18895 PMID 11121026 . Hinkley CS, Hirsch HA, Gu L, et al. (2003). "The small nuclear RNA-activating protein 190 Myb DNA binding domain stimulates TATA box-binding protein-TATA box recognition" . J. Biol. Chem . 278 (20): 18649–57. doi :10.1074/jbc.M204247200 PMID 12621023 . Saxena A, Ma B, Schramm L, Hernandez N (2005). "Structure-function analysis of the human TFIIB-related factor II protein reveals an essential role for the C-terminal domain in RNA polymerase III transcription" . Mol. Cell. Biol . 25 (21): 9406–18. doi :10.1128/MCB.25.21.9406-9418.2005 . PMC 1265830 PMID 16227591 .