GLG1
Protein 1 Golgijevog aparata je protein koji je kod ljudi kodiran genom GLG1.[5][6][7]
Aminokiselinska sekvenca
Dužina polipeptidnog lanca je 1.179 aminokiselina, a molekulska težina 134.552 Da.[8].
- Simboli
C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MAACGRVRRM | FRLSAALHLL | LLFAAGAEKL | PGQGVHSQGQ | GPGANFVSFV | ||||
| GQAGGGGPAG | QQLPQLPQSS | QLQQQQQQQQ | QQQQPQPPQP | PFPAGGPPAR | ||||
| RGGAGAGGGW | KLAEEESCRE | DVTRVCPKHT | WSNNLAVLEC | LQDVREPENE | ||||
| ISSDCNHLLW | NYKLNLTTDP | KFESVAREVC | KSTITEIKEC | ADEPVGKGYM | ||||
| VSCLVDHRGN | ITEYQCHQYI | TKMTAIIFSD | YRLICGFMDD | CKNDINILKC | ||||
| GSIRLGEKDA | HSQGEVVSCL | EKGLVKEAEE | REPKIQVSEL | CKKAILRVAE | ||||
| LSSDDFHLDR | HLYFACRDDR | ERFCENTQAG | EGRVYKCLFN | HKFEESMSEK | ||||
| CREALTTRQK | LIAQDYKVSY | SLAKSCKSDL | KKYRCNVENL | PRSREARLSY | ||||
| LLMCLESAVH | RGRQVSSECQ | GEMLDYRRML | MEDFSLSPEI | ILSCRGEIEH | ||||
| HCSGLHRKGR | TLHCLMKVVR | GEKGNLGMNC | QQALQTLIQE | TDPGADYRID | ||||
| RALNEACESV | IQTACKHIRS | GDPMILSCLM | EHLYTEKMVE | DCEHRLLELQ | ||||
| YFISRDWKLD | PVLYRKCQGD | ASRLCHTHGW | NETSEFMPQG | AVFSCLYRHA | ||||
| YRTEEQGRRL | SRECRAEVQR | ILHQRAMDVK | LDPALQDKCL | IDLGKWCSEK | ||||
| TETGQELECL | QDHLDDLVVE | CRDIVGNLTE | LESEDIQIEA | LLMRACEPII | ||||
| QNFCHDVADN | QIDSGDLMEC | LIQNKHQKDM | NEKCAIGVTH | FQLVQMKDFR | ||||
| FSYKFKMACK | EDVLKLCPNI | KKKVDVVICL | STTVRNDTLQ | EAKEHRVSLK | ||||
| CRRQLRVEEL | EMTEDIRLEP | DLYEACKSDI | KNFCSAVQYG | NAQIIECLKE | ||||
| NKKQLSTRCH | QKVFKLQETE | MMDPELDYTL | MRVCKQMIKR | FCPEADSKTM | ||||
| LQCLKQNKNS | ELMDPKCKQM | ITKRQITQNT | DYRLNPMLRK | ACKADIPKFC | ||||
| HGILTKAKDD | SELEGQVISC | LKLRYADQRL | SSDCEDQIRI | IIQESALDYR | ||||
| LDPQLQLHCS | DEISSLCAEE | AAAQEQTGQV | EECLKVNLLK | IKTELCKKEV | ||||
| LNMLKESKAD | IFVDPVLHTA | CALDIKHHCA | AITPGRGRQM | SCLMEALEDK | ||||
| RVRLQPECKK | RLNDRIEMWS | YAAKVAPADG | FSDLAMQVMT | SPSKNYILSV | ||||
| ISGSICILFL | IGLMCGRITK | RVTRELKDR |
Kloniranje i ekspresija
[uredi | uredi izvor]Mourelatos et al. (1996) izolovali su i okarakterisali ljudske GLG1 cDNK. cDNK pune dužine kodira predviđeni protein od 1179 aminokiselina, a koji ima signalni peptid, regiju bogatu prolinom i glutaminom, domen bogat cisteinom koji sadrži pet potencijalnih mjesta glikozilacije povezane asparaginom, hidrofobni transmembranski domen i kratki karboksi-terminalni rep. Segment bogat prolinom i glutaminom sadrži poliglutaminsku dionicu koja je kodirana neprekinutom egzonskom sekvencom ponavljanja CAG-CAA. Ljudski GLG1 pokazuje između 88% i 95% ukupnog identiteta aminokiselina sa Glg1 pacova, Esl1 miša i CRF kokoši. Autori su Northern blot analizom otkrili tri transkripta od 10, 5 i 3,8 kb u nekoliko ljudskih tkiva fetusa i odraslih.
Funkcija gena
[uredi | uredi izvor]MG-160, izvorno izoliran iz mozga pacova, konzervirani je membranski sijaloglikoprotein u medijalnim cisternama Golgijevog aparata većine ćelija (Gonatas et al., 1989).[9] MG-160 se pojavljuje rano u u razvoju kokošijeg embriona i perzistira u većini ćelijaa tokom embrionskg razvoja i kod odrasle životinje (Mourelatos et al., 1995).[10][11] Rano pojavljivanje i sveprisutnost MG-160 sugeriralo je da protein ima važnu ulogu u biogenezi i funkciji Golgijevog aparata. MG-160 veže osnovni faktor rasta fibroblasta (FGFB) i pokazuje 90% identiteta aminokiselina sa CFR, receptorom bogatim cisteinom za nekoliko faktora rasta fibroblasta koji je izoliran iz kokošijeg embriona. Za razliku od receptora faktora rasta fibroblasta koji su uključeni u transdukciju signala, MG-160 i njegov kokošiji homolog, CFR, ne pokazuju vanćelijske obrasce slične Ig i domene intracitoplazmatske tirozin-kinaze. Pored CFR-a, MG-160 je vrlo homologan ESL-1, ligandu za E-selektina (SELE; 131210).
Struktura gena
[uredi | uredi izvor]Willmroth i Beaudet (1999) utvrdili su da mišji miš Esl1 sadrži 27 egzona i da se proteže preko 75 kb.
Mapiranje
[uredi | uredi izvor]Mourelatos et al. (1995), fluorescentnom in situ hibridizacijom, lokalizirali su ljudski GLG1 gen za MG-160 u sekvenci 16q22-q23. Ako MG-160, kako je pretpostavljeno, posreduje u lučenju faktora rasta fibroblasta, hromomska lokalizacija gena može olakšati razumijevanje uloge gena u ljudskoj bolesti. Southern blot analizom interspecifičnog unatražno ukrštajućeg panela Willmroth i Beaudet (1999) mapirali i su Esl1, mišji homolog gena GLG1, na hromosom 8, u regiji koja pokazuje homologiju sinteze sa ljudskim 16q.[12]
Reference
[uredi | uredi izvor]- ^ a b c GRCh38: Ensembl release 89: ENSG00000090863 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000003316 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Mourelatos Z, Gonatas JO, Nycum LM, Gonatas NK, Biegel JA (Feb 1996). "Assignment of the GLG1 gene for MGF-160, a fibroblast growth factor and E-selectin binding membrane sialoglycoprotein of the Golgi apparatus, to chromosome 16q22-q23 by fluorescence in situ hybridization". Genomics. 28 (2): 354–5. doi:10.1006/geno.1995.1156. PMID 8530051.
- ^ Steegmaier M, Levinovitz A, Isenmann S, Borges E, Lenter M, Kocher HP, Kleuser B, Vestweber D (Mar 1995). "The E-selectin-ligand ESL-1 is a variant of a receptor for fibroblast growth factor". Nature. 373 (6515): 615–20. doi:10.1038/373615a0. PMID 7531823. S2CID 1602373.
- ^ "Entrez Gene: GLG1 golgi apparatus protein 1".
- ^ "UniProt, Q92896". Pristupljeno 6. 7. 2021.
- ^ Gonatas, J. O., Mezitis, S. G. E., Stieber, A., Fleischer, B., Gonatas, N. K. MG-160. A novel sialoglycoprotein of the medical cisternae of the Golgi apparatus. J. Biol. Chem. 264: 646-653, 1989. [+PubMed: 2909545
- ^ Mourelatos, Z., Gonatas, J. O., Cinato, E., Gonatas, N. K. Cloning and sequence analysis of the human MG160, a fibroblast growth factor and E-selectin binding membrane sialoglycoprotein of the Golgi apparatus. DNA Cell Biol. 15: 1121-1128, 1996. PubMed: 8985126
- ^ Mourelatos, Z., Gonatas, J. O., Nycum, L. M., Gonatas, N. K., Biegel, J. A. Assignment of the GLG1 gene for MG-160, a fibroblast growth factor and E-selectin binding membrane sialoglycoprotein of the Golgi apparatus, to chromosome 16q22-q23 by fluorescence in situ hybridization. Genomics 28: 354-355, 1995. PubMed: 8530051
- ^ Willmroth, F., Beaudet, A. L. Structure of the murine E-selectin ligand 1 (ESL-1) gene and assignment to chromosome 8. Mammalian Genome 10: 1085-1088, 1999. PubMed: 10556428
Dopunska literatura
[uredi | uredi izvor]- Burrus LW, Zuber ME, Lueddecke BA, Olwin BB (1992). "Identification of a cysteine-rich receptor for fibroblast growth factors". Mol. Cell. Biol. 12 (12): 5600–9. doi:10.1128/MCB.12.12.5600. PMC 360499. PMID 1448090.
- Croul S, Mezitis SG, Stieber A, et al. (1990). "Immunocytochemical visualization of the Golgi apparatus in several species, including human, and tissues with an antiserum against MG-160, a sialoglycoprotein of rat Golgi apparatus". J. Histochem. Cytochem. 38 (7): 957–63. doi:10.1177/38.7.2355176. PMID 2355176.
- Mourelatos Z, Gonatas JO, Cinato E, Gonatas NK (1997). "Cloning and sequence analysis of the human MG160, a fibroblast growth factor and E-selectin binding membrane sialoglycoprotein of the Golgi apparatus". DNA Cell Biol. 15 (12): 1121–8. doi:10.1089/dna.1996.15.1121. PMID 8985126.
- Steegmaier M, Borges E, Berger J, et al. (1997). "The E-selectin-ligand ESL-1 is located in the Golgi as well as on microvilli on the cell surface". J. Cell Sci. 110 (6): 687–94. PMID 9099943.
- Olofsson A, Hellman U, Ten Dijke P, et al. (1997). "Latent transforming growth factor-beta complex in Chinese hamster ovary cells contains the multifunctional cysteine-rich fibroblast growth factor receptor, also termed E-selectin-ligand or MG-160". Biochem. J. 324 (Pt 2): 427–34. doi:10.1042/bj3240427. PMC 1218448. PMID 9182700.
- Wild MK, Huang MC, Schulze-Horsel U, et al. (2001). "Affinity, kinetics, and thermodynamics of E-selectin binding to E-selectin ligand-1". J. Biol. Chem. 276 (34): 31602–12. doi:10.1074/jbc.M104844200. PMID 11404363.
- Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
- Yamaguchi F, Morrison RS, Gonatas NK, et al. (2003). "Identification of MG-160, a FGF binding medial Golgi sialoglycoprotein, in brain tumors: an index of malignancy in astrocytomas". Int. J. Oncol. 22 (5): 1045–9. doi:10.3892/ijo.22.5.1045. PMID 12684670.
- Zhang H, Li XJ, Martin DB, Aebersold R (2003). "Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry". Nat. Biotechnol. 21 (6): 660–6. doi:10.1038/nbt827. PMID 12754519. S2CID 581283.
- Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
- Bouwmeester T, Bauch A, Ruffner H, et al. (2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol. 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216. S2CID 11683986.
- Ahn J, Febbraio M, Silverstein RL (2005). "A novel isoform of human Golgi complex-localized glycoprotein-1 (also known as E-selectin ligand-1, MG-160 and cysteine-rich fibroblast growth factor receptor) targets differential subcellular localization". J. Cell Sci. 118 (Pt 8): 1725–31. doi:10.1242/jcs.02310. PMID 15797922.