MCM5

MCM5
Identifikatori
Aliasi	MCM5
Vanjski ID-jevi	OMIM: 602696 MGI: 103197 HomoloGene: 4904 GeneCards: MCM5
Lokacija gena (čovjek) Hrom. Hromosom 22 (čovjek)[1] Bend 22q12.3 Početak 35,400,134 bp[1] Kraj 35,425,431 bp[1]
Lokacija gena (miš) Hrom. Hromosom 8 (miš)[2] Bend 8|8 C1 Početak 75,836,197 bp[2] Kraj 75,855,067 bp[2]
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • nucleotide binding • vezivanje sa DNK • GO:0004003 DNA helicase activity • DNA replication origin binding • chromatin binding • GO:0001948, GO:0016582 vezivanje za proteine • hydrolase activity • ATP binding • GO:0008026 helicase activity • single-stranded DNA binding • GO:0043142 single-stranded DNA helicase activity • GO:0043140 3'-5' DNA helicase activity Ćelijska komponenta • MCM complex • membrana • jedro • nukleoplazma • citoplazma • citosol Biološki proces • G1 faza • Replikacija DNK • ćelijski ciklus • DNA duplex unwinding • DNA replication initiation • G1/S transition of mitotic cell cycle • double-strand break repair via break-induced replication • pre-replicative complex assembly involved in nuclear cell cycle DNA replication Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	4174	17218
Ensembl	ENSG00000100297	ENSMUSG00000005410
UniProt	P33992	P49718
RefSeq (mRNK)	NM_006739	NM_008566 NM_001302540
RefSeq (bjelančevina)	NP_006730	NP_001289469 NP_032592
Lokacija (UCSC)	Chr 22: 35.4 – 35.43 Mb	Chr 8: 75.84 – 75.86 Mb
PubMed pretraga	[3]	[4]
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

MCM5 faktor licenciranja replikacije DNK jest protein koji je kod ljudi kodiran genom MCM5 sa hromosoma 22.^[5][6][7]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 734 aminokiseline, a molekulska težina 82.286 Da.^[7]

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

10		20		30		40		50
MSGFDDPGIF		YSDSFGGDAQ		ADEGQARKSQ		LQRRFKEFLR		QYRVGTDRTG
FTFKYRDELK		RHYNLGEYWI		EVEMEDLASF		DEDLADYLYK		QPAEHLQLLE
EAAKEVADEV		TRPRPSGEEV		LQDIQVMLKS		DASPSSIRSL		KSDMMSHLVK
IPGIIIAASA		VRAKATRISI		QCRSCRNTLT		NIAMRPGLEG		YALPRKCNTD
QAGRPKCPLD		PYFIMPDKCK		CVDFQTLKLQ		ELPDAVPHGE		MPRHMQLYCD
RYLCDKVVPG		NRVTIMGIYS		IKKFGLTTSR		GRDRVGVGIR		SSYIRVLGIQ
VDTDGSGRSF		AGAVSPQEEE		EFRRLAALPN		VYEVISKSIA		PSIFGGTDMK
KAIACLLFGG		SRKRLPDGLT		RRGDINLLML		GDPGTAKSQL		LKFVEKCSPI
GVYTSGKGSS		AAGLTASVMR		DPSSRNFIME		GGAMVLADGG		VVCIDEFDKM
REDDRVAIHE		AMEQQTISIA		KAGITTTLNS		RCSVLAAANS		VFGRWDETKG
EDNIDFMPTI		LSRFDMIFIV		KDEHNEERDV		MLAKHVITLH		VSALTQTQAV
EGEIDLAKLK		KFIAYCRVKC		GPRLSAEAAE		KLKNRYIIMR		SGARQHERDS
DRRSSIPITV		RQLEAIVRIA		EALSKMKLQP		FATEADVEEA		LRLFQVSTLD
AALSGTLSGV		EGFTSQEDQE		MLSRIEKQLK		RRFAIGSQVS		EHSIIKDFTK
QKYPEHAIHK		VLQLMLRRGE		IQHRMQRKVL		YRLK

Funkcija

Protein kodiran ovim genom je strukturno vrlo sličan proteinu CDC46 iz S. cerevisiae, uključenom u inicijaciju replikacije DNK. Kodirani protein je član porodice proteina MCM koji se vezuju za hromatin i može komunicirati sa najmanje dva druga člana ove porodice. Kodirani protein se pojačava u tranziciji iz G0 u G1/S fazu ćelijskog ciklusa i može aktivno učestvovati u regulaciji ćelijskog ciklusa.^[7]

Interakcije

Pokazano je da MCM5 ima interakcije sa:

• PKBCD7,^[8]
• MCM2,^[8][9]
• MCM3,^[8][9]
• MCM7,^[8][9][10]
• ORC2L,^[8]
• ORC6L,^[8] i
• STAT1.^[9][11]

Reference

1. GRCh38: Ensembl release 89: ENSG00000100297 - Ensembl, maj 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000005410 - Ensembl, maj 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Paul R, Hu B, Musahl C, Hameister H, Knippers R (Sep 1996). "Coding sequence and chromosome mapping of the human gene (CDC46) for replication protein hCdc46/Mcm5". Cytogenet Cell Genet. 73 (4): 317–21. doi:10.1159/000134366. PMID 8751386.
6. Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M, Smink LJ, Ainscough R, Almeida JP, Babbage A, Bagguley C, Bailey J, Barlow K, Bates KN, Beasley O, Bird CP, Blakey S, Bridgeman AM, Buck D, Burgess J, Burrill WD, O'Brien KP (Dec 1999). "The DNA sequence of human chromosome 22". Nature. 402 (6761): 489–95. Bibcode:1999Natur.402..489D. doi:10.1038/990031. PMID 10591208.
7. "Entrez Gene: MCM5 MCM5 minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae)".
8. Kneissl M, Pütter V, Szalay AA, Grummt F (Mar 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1): 111–28. doi:10.1016/s0022-2836(03)00079-2. PMID 12614612.
9. DaFonseca CJ, Shu F, Zhang JJ (Mar 2001). "Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma". Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3034–9. Bibcode:2001PNAS...98.3034D. doi:10.1073/pnas.061487598. PMC 30602. PMID 11248027.
10. Fujita M, Kiyono T, Hayashi Y, Ishibashi M (Apr 1997). "In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP". J. Biol. Chem. 272 (16): 10928–35. doi:10.1074/jbc.272.16.10928. PMID 9099751.
11. Zhang JJ, Zhao Y, Chait BT, Lathem WW, Ritzi M, Knippers R, Darnell JE (Dec 1998). "Ser727-dependent recruitment of MCM5 by Stat1alpha in IFN-gamma-induced transcriptional activation". EMBO J. 17 (23): 6963–71. doi:10.1093/emboj/17.23.6963. PMC 1171044. PMID 9843502.

Dopunska literatura

• Burkhart R, Schulte D, Hu D, Musahl C, Göhring F, Knippers R (1995). "Interactions of human nuclear proteins P1Mcm3 and P1Cdc46". Eur. J. Biochem. 228 (2): 431–8. doi:10.1111/j.1432-1033.1995.tb20281.x. PMID 7705359.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Hu B, Burkhart R, Schulte D, Musahl C, Knippers R (1994). "The P1 family: a new class of nuclear mammalian proteins related to the yeast Mcm replication proteins". Nucleic Acids Res. 21 (23): 5289–93. doi:10.1093/nar/21.23.5289-a. PMC 310560. PMID 8265339.
• Ishimi Y, Ichinose S, Omori A, Sato K, Kimura H (1996). "Binding of human minichromosome maintenance proteins with histone H3". J. Biol. Chem. 271 (39): 24115–22. doi:10.1074/jbc.271.39.24115. PMID 8798650.
• Fujita M, Kiyono T, Hayashi Y, Ishibashi M (1997). "In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP". J. Biol. Chem. 272 (16): 10928–35. doi:10.1074/jbc.272.16.10928. PMID 9099751.
• Tsuruga H, Yabuta N, Hashizume K, Ikeda M, Endo Y, Nojima H (1997). "Expression, nuclear localization and interactions of human MCM/P1 proteins". Biochem. Biophys. Res. Commun. 236 (1): 118–25. doi:10.1006/bbrc.1997.6865. PMID 9223437.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Zhang JJ, Zhao Y, Chait BT, Lathem WW, Ritzi M, Knippers R, Darnell JE (1999). "Ser727-dependent recruitment of MCM5 by Stat1alpha in IFN-gamma-induced transcriptional activation". EMBO J. 17 (23): 6963–71. doi:10.1093/emboj/17.23.6963. PMC 1171044. PMID 9843502.
• Ohtani K, Iwanaga R, Nakamura M, Ikeda M, Yabuta N, Tsuruga H, Nojima H (1999). "Cell growth-regulated expression of mammalian MCM5 and MCM6 genes mediated by the transcription factor E2F". Oncogene. 18 (14): 2299–309. doi:10.1038/sj.onc.1202544. PMID 10327050.
• DaFonseca CJ, Shu F, Zhang JJ (2001). "Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma". Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3034–9. Bibcode:2001PNAS...98.3034D. doi:10.1073/pnas.061487598. PMC 30602. PMID 11248027.
• Going JJ, Keith WN, Neilson L, Stoeber K, Stuart RC, Williams GH (2002). "Aberrant expression of minichromosome maintenance proteins 2 and 5, and Ki-67 in dysplastic squamous oesophageal epithelium and Barrett's mucosa". Gut. 50 (3): 373–7. doi:10.1136/gut.50.3.373. PMC 1773132. PMID 11839717.
• Kneissl M, Pütter V, Szalay AA, Grummt F (2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1): 111–28. doi:10.1016/S0022-2836(03)00079-2. PMID 12614612.
• Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G (2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol. 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216. S2CID 11683986.
• Ramachandran N, Hainsworth E, Bhullar B, Eisenstein S, Rosen B, Lau AY, Walter JC, LaBaer J (2004). "Self-assembling protein microarrays". Science. 305 (5680): 86–90. Bibcode:2004Sci...305...86R. doi:10.1126/science.1097639. PMID 15232106. S2CID 20936301.
• Yu Z, Feng D, Liang C (2004). "Pairwise interactions of the six human MCM protein subunits". J. Mol. Biol. 340 (5): 1197–206. doi:10.1016/j.jmb.2004.05.024. PMID 15236977.
• Jin J, Smith FD, Stark C, Wells CD, Fawcett JP, Kulkarni S, Metalnikov P, O'Donnell P, Taylor P, Taylor L, Zougman A, Woodgett JR, Langeberg LK, Scott JD, Pawson T (2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Curr. Biol. 14 (16): 1436–50. doi:10.1016/j.cub.2004.07.051. PMID 15324660. S2CID 2371325.