PDP1

PDP1
Identifikatori
Aliasi	PDP1
Vanjski ID-jevi	OMIM: 605993 MGI: 2685870 HomoloGene: 31928 GeneCards: PDP1
Lokacija gena (čovjek) Hrom. Hromosom 8 (čovjek)[1] Bend 8q22.1 Početak 93,857,807 bp[1] Kraj 93,926,068 bp[1]
Lokacija gena (miš) Hrom. Hromosom 4 (miš)[2] Bend 4|4 A1 Početak 11,958,184 bp[2] Kraj 11,966,452 bp[2]
Ontologija gena Molekularna funkcija • protein serine/threonine phosphatase activity • GO:0001948, GO:0016582 vezivanje za proteine • catalytic activity • phosphoprotein phosphatase activity • hydrolase activity • cation binding • vezivanje iona metala • [pyruvate dehydrogenase (lipoamide) phosphatase activity] Ćelijska komponenta • mitochondrial matrix • mitohondrija Biološki proces • GO:0016576 protein dephosphorylation • regulation of acetyl-CoA biosynthetic process from pyruvate • peptidyl-threonine dephosphorylation • positive regulation of pyruvate dehydrogenase activity Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	54704	381511
Ensembl	ENSG00000164951	ENSMUSG00000049225
UniProt	Q9P0J1	Q3UV70
RefSeq (mRNK)	NM_001161778 NM_001161779 NM_001161780 NM_001161781 NM_018444	NM_001033453 NM_001098230 NM_001098231 NM_001290387 NM_001290391
RefSeq (bjelančevina)	NP_001155251 NP_001155252 NP_001155253 NP_060914	NP_001028625 NP_001091700 NP_001091701 NP_001277316 NP_001277320
Lokacija (UCSC)	Chr 8: 93.86 – 93.93 Mb	Chr 4: 11.96 – 11.97 Mb
PubMed pretraga	[3]	[4]
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Katalitska podjedinica 1 piruvat-dehidrogenaze (PDPC 1), znana i kao protein-fosfataza 2C, je enzim koji je kod ljudi kodiran genom PDP1.^[5][6] PDPC 1 je enzim koji pruža povratne efrkte piruvat-dehidrogenaznih kinaza nakon piruvat-dehidrogenaze, aktivirajući piruvat dehidrogenazu.

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 537 aminokiselina, а molekulska težina 61.054 Da.^[7].

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

10		20		30		40		50
MPAPTQLFFP		LIRNCELSRI		YGTACYCHHK		HLCCSSSYIP		QSRLRYTPHP
AYATFCRPKE		NWWQYTQGRR		YASTPQKFYL		TPPQVNSILK		ANEYSFKVPE
FDGKNVSSIL		GFDSNQLPAN		APIEDRRSAA		TCLQTRGMLL		GVFDGHAGCA
CSQAVSERLF		YYIAVSLLPH		ETLLEIENAV		ESGRALLPIL		QWHKHPNDYF
SKEASKLYFN		SLRTYWQELI		DLNTGESTDI		DVKEALINAF		KRLDNDISLE
AQVGDPNSFL		NYLVLRVAFS		GATACVAHVD		GVDLHVANTG		DSRAMLGVQE
EDGSWSAVTL		SNDHNAQNER		ELERLKLEHP		KSEAKSVVKQ		DRLLGLLMPF
RAFGDVKFKW		SIDLQKRVIE		SGPDQLNDNE		YTKFIPPNYH		TPPYLTAEPE
VTYHRLRPQD		KFLVLATDGL		WETMHRQDVV		RIVGEYLTGM		HHQQPIAVGG
YKVTLGQMHG		LLTERRTKMS		SVFEDQNAAT		HLIRHAVGNN		EFGTVDHERL
SKMLSLPEEL		ARMYRDDITI		IVVQFNSHVV		GAYQNQE

Funkcija

Piruvat-dehidrogenaza (E1) jedna je od tri komponente (E1, E2 i E3) velikog kompleksa piruvat-dehidrogenaza. Piruvat-dehidrogenazne kinaze kataliziraju fosforilaciju ostataka serina E1 da inaktiviraju komponentu E1 i inhibiraju kompleks. Fosfataze piruvat-dehidrogenaze kataliziraju defosforilaciju i aktivaciju komponente E1 za poništavanje učinaka piruvat-dehidrogenaznih kinaza.

Piruvat-dehidrogenazna fosfataza je heterodimer koji se sastoji od katalitske i regulatorne podjedinice. Prijavljene su dvije katalitske podjedinice; jedna je pretežno ekaprimirana u skeletnim mišićima, a druga je mnogo obilnija u jetri. Katalitska podjedinica, kodirana ovim genom, prva je i pripada superporodici proteinskih fosfataza 2C (PP2C). Uz kompleks piruvat-dehidrogenaze i piruvat-dehidrogenazne kinaze, ovaj enzim se nalazi u mitohondrijskom matriksu.^[5]

Regulacija

Fosfatazu piruvat-dehidrogenaze stimuliraju insulin, PEP i AMP, ali je kompetitivno inhibirana pomoću ATP , Nikotinamid adenin-dinukleotida (NADH-a) i Acetil-CoA.

Klinički značaj

Mutacija u ovom genu uzrokuje nedostatak piruvat-dehidrogenazne fosfataze.^[5]

Reference

1. GRCh38: Ensembl release 89: ENSG00000164951 - Ensembl, maj 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000049225 - Ensembl, maj 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: pyruvate dehydrogenase phosphatase catalytic subunit 1".
6. Lawson JE, Niu XD, Browning KS, Trong HL, Yan J, Reed LJ (Sep 1993). "Molecular cloning and expression of the catalytic subunit of bovine pyruvate dehydrogenase phosphatase and sequence similarity with protein phosphatase 2C". Biochemistry. 32 (35): 8987–93. doi:10.1021/bi00086a002. PMID 8396421.
7. "UniProt, Q9P0J1" (jezik: engleski). Pristupljeno 20. 9. 2021.

Dopunska litErarur

• Piccinini M, Mostert M, Alberto G, Ramondetti C, Novi RF, Dalmasso P, Rinaudo MT (Apr 2005). "Down-regulation of pyruvate dehydrogenase phosphatase in obese subjects is a defect that signals insulin resistance". Obesity Research. 13 (4): 678–86. doi:10.1038/oby.2005.76. PMID 15897476.
• Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S (Jan 2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Research. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
• Hu RM, Han ZG, Song HD, Peng YD, Huang QH, Ren SX, Gu YJ, Huang CH, Li YB, Jiang CL, Fu G, Zhang QH, Gu BW, Dai M, Mao YF, Gao GF, Rong R, Ye M, Zhou J, Xu SH, Gu J, Shi JX, Jin WR, Zhang CK, Wu TM, Huang GY, Chen Z, Chen MD, Chen JL (Aug 2000). "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning". Proceedings of the National Academy of Sciences of the United States of America. 97 (17): 9543–8. Bibcode:2000PNAS...97.9543H. doi:10.1073/pnas.160270997. PMC 16901. PMID 10931946.
• Auffray C, Behar G, Bois F, Bouchier C, Da Silva C, Devignes MD, Duprat S, Houlgatte R, Jumeau MN, Lamy B (Feb 1995). "[IMAGE: molecular integration of the analysis of the human genome and its expression]". Comptes Rendus de l'Académie des Sciences, Série III. 318 (2): 263–72. PMID 7757816.
• Lejeune F, Li X, Maquat LE (Sep 2003). "Nonsense-mediated mRNA decay in mammalian cells involves decapping, deadenylating, and exonucleolytic activities". Molecular Cell. 12 (3): 675–87. doi:10.1016/S1097-2765(03)00349-6. PMID 14527413.
• Cameron JM, Maj M, Levandovskiy V, Barnett CP, Blaser S, Mackay N, Raiman J, Feigenbaum A, Schulze A, Robinson BH (Apr 2009). "Pyruvate dehydrogenase phosphatase 1 (PDP1) null mutation produces a lethal infantile phenotype". Human Genetics. 125 (3): 319–26. doi:10.1007/s00439-009-0629-6. PMID 19184109. S2CID 20251714.
• Maj MC, MacKay N, Levandovskiy V, Addis J, Baumgartner ER, Baumgartner MR, Robinson BH, Cameron JM (Jul 2005). "Pyruvate dehydrogenase phosphatase deficiency: identification of the first mutation in two brothers and restoration of activity by protein complementation". The Journal of Clinical Endocrinology and Metabolism. 90 (7): 4101–7. doi:10.1210/jc.2005-0123. PMID 15855260.
• Sugden MC, Holness MJ (maj 2003). "Recent advances in mechanisms regulating glucose oxidation at the level of the pyruvate dehydrogenase complex by PDKs". American Journal of Physiology. Endocrinology and Metabolism. 284 (5): E855-62. doi:10.1152/ajpendo.00526.2002. PMID 12676647.
• Kato J, Kato M (Mar 2010). "Crystallization and preliminary crystallographic studies of the catalytic subunits of human pyruvate dehydrogenase phosphatase isoforms 1 and 2". Acta Crystallographica Section F. 66 (Pt 3): 342–5. doi:10.1107/S1744309110003131. PMC 2833053. PMID 20208177.
• Korotchkina LG, Patel MS (Jun 1995). "Mutagenesis studies of the phosphorylation sites of recombinant human pyruvate dehydrogenase. Site-specific regulation". The Journal of Biological Chemistry. 270 (24): 14297–304. doi:10.1074/jbc.270.24.14297. PMID 7782287.
• Stellingwerff T, Spriet LL, Watt MJ, Kimber NE, Hargreaves M, Hawley JA, Burke LM (Feb 2006). "Decreased PDH activation and glycogenolysis during exercise following fat adaptation with carbohydrate restoration". American Journal of Physiology. Endocrinology and Metabolism. 290 (2): E380-8. doi:10.1152/ajpendo.00268.2005. PMID 16188909.
• Ito M, Kobashi H, Naito E, Saijo T, Takeda E, Huq AH, Kuroda Y (Jul 1992). "Decrease of pyruvate dehydrogenase phosphatase activity in patients with congenital lactic acidemia". Clinica Chimica Acta; International Journal of Clinical Chemistry. 209 (1–2): 1–7. doi:10.1016/0009-8981(92)90327-M. PMID 1327585.
• Adams MD, Kerlavage AR, Fleischmann RD, Fuldner RA, Bult CJ, Lee NH, Kirkness EF, Weinstock KG, Gocayne JD, White O (Sep 1995). "Initial assessment of human gene diversity and expression patterns based upon 83 million nucleotides of cDNA sequence". Nature. 377 (6547 Suppl): 3–174. PMID 7566098.
• Caruso M, Maitan MA, Bifulco G, Miele C, Vigliotta G, Oriente F, Formisano P, Beguinot F (Nov 2001). "Activation and mitochondrial translocation of protein kinase Cdelta are necessary for insulin stimulation of pyruvate dehydrogenase complex activity in muscle and liver cells". The Journal of Biological Chemistry. 276 (48): 45088–97. doi:10.1074/jbc.M105451200. PMID 11577086.
• Bonaldo MF, Lennon G, Soares MB (Sep 1996). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Research. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Huang B, Gudi R, Wu P, Harris RA, Hamilton J, Popov KM (Jul 1998). "Isoenzymes of pyruvate dehydrogenase phosphatase. DNA-derived amino acid sequences, expression, and regulation". The Journal of Biological Chemistry. 273 (28): 17680–8. doi:10.1074/jbc.273.28.17680. PMID 9651365.

Vanjski linkovi

• Pyruvate Dehydrogenase Phosphatase na US National Library of Medicine Medical Subject Headings (MeSH)

Ovaj članak uključuje tekst iz Nacionalne medicinske biblioteke Sjedinjenih Država, koji je u javnom vlasništvu.