SDPR

SDPR
Identifikatori
Aliasi	CAVIN2
Vanjski ID-jevi	OMIM: 606728 MGI: 99513 HomoloGene: 3422 GeneCards: CAVIN2
Lokacija gena (čovjek)
Hrom.	Hromosom 2 (čovjek)
Kraj	191,847,088 bp
Lokacija gena (miš)
Hrom.	Hromosom 1 (miš)
Kraj	51,342,119 bp
Obrazac RNK ekspresije
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• phosphatidylserine binding; • GO:0001948, GO:0016582 vezivanje za proteine; • phospholipid binding; • protein kinase C binding; • lipid binding;
Ćelijska komponenta	• citoplazma; • Lipidni splav; • citosol; • membrana; • Kaveole; • nukleoplazma; • ćelijska membrana; • actin cytoskeleton;
Biološki proces	• plasma membrane tubulation;
	Izvori:Amigo / QuickGO
Ortolozi
	8436
	20324
	ENSG00000168497
	ENSMUSG00000045954
	O95810
	Q63918
	NM_004657
	NM_138741
	NP_004648
	NP_620080
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Kavin-2 protein odgovora na deprivaciju seruma (SDPR) jest protein koji je kod ljudi kodiran genom SDPR sa hromosoma 2^[5]^[6]^[7] Kavin-2 je visoko eksprimiran u raznim ljudskum endotelnim ćelijama.^[8]

Ovaj gen kodira kalcij nezavisan fosfolipid-vezujući protein čija se ekspresija povećava u serumu izgladnjelii ćelija. Također se pokazalo da je ovaj protein supstrat za fosforilacije protein kinaza C (PKC).^[7]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 425 aminokiselina, a molekulska težina 47.173 Da.^[9]

C: Cistein

D: Asparaginska kiselina

E: Glutaminska kiselina

F: Fenilalanin

G: Glicin

H: Histidin

I: Izoleucin

K: Lizin

L: Leucin

M: Metionin

N: Asparagin

P: Prolin

Q: Glutamin

R: Arginin

S: Serin

T: Treonin

V: Valin

W: Triptofan

Y: Tirozin

10	20	30	40	50
MGEDAAQAEK	FQHPGSDMRQ	EKPSSPSPMP	SSTPSPSLNL	GNTEEAIRDN
SQVNAVTVLT	LLDKLVNMLD	AVQENQHKME	QRQISLEGSV	KGIQNDLTKL
SKYQASTSNT	VSKLLEKSRK	VSAHTRAVKE	RMDRQCAQVK	RLENNHAQLL
RRNHFKVLIF	QEENEIPASV	FVKQPVSGAV	EGKEELPDEN	KSLEETLHTV
DLSSDDDLPH	DEEALEDSAE	EKVEESRAEK	IKRSSLKKVD	SLKKAFSRQN
IEKKMNKLGT	KIVSVERREK	IKKSLTSNHQ	KISSGKSSPF	KVSPLTFGRK
KVREGESHAE	NETKSEDLPS	SEQMPNDQEE	ESFAEGHSEA	SLASALVEGE
IAEEAAEKAT	SRGSNSGMDS	NIDLTIVEDE	EEESVALEQA	QKVRYEGSYA
LTSEEAERSD	GDPVQPAVLQ	VHQTS

Funkcija

Kavin-2 je neophodan za formiranje krvnih sudova (angiogenezu) kod ljudi i zebrica, a također je potreban i za proliferaciju endotelnih ćelija, migracijku i invaziju kod ljudi.^[8] Kavin-2 ima važnu ulogu u endotelnim ćelijama, održavanjem regulacije aktivnosti eNOS-a.^[8] Kavin-2 kontrolira stvaranje oksid[a (NO) u ljudskim endotelnim ćelijama, kontroliranjem aktivnosti i stabilnosti proteina endotelne dušik-oksidne sintaze (eNOS).^[8]

Sekrecija

Kavin-2 se visoko luči iz ljudskih endotelnih ćelija (HUVEC), luče se kroz endotelne mikročestice (EMP), ali ne egzozome i potrebna je za biogenezu EMP^[8]

Klinički značaj

Pokazalo se da SDPR djeluje kao supresor metastaza u ksenotransplantatu , u studijama koje koriste ćelijske linije raka dojke.^[10]

SDPR može izazvati funkciju supresora metastaza direktnom interakcijom sa ERK i ograničavanjem njegove uloge za preživljavanje.^[10] Štaviše, sugerira se da se SDPR utišava tokom progresija raka dojke putem promotorske DNK-metilacije.^[10] Uloga SDPR-a supresora metastaza može ići dalje od raka dojke jer uzorci tumora iz mokraćne bešike, kolorektunskog crieva, pluća, pankreasa , a rak jajnika kao i sarkomi također su pokazali gubitak ekspresije SDPR-a.^[10]

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000168497 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000045954 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Gustincich S, Vatta P, Goruppi S, Wolf M, Saccone S, Della Valle G, et al. (april 1999). "The human serum deprivation response gene (SDPR) maps to 2q32-q33 and codes for a phosphatidylserine-binding protein". Genomics. 57 (1): 120–9. doi:10.1006/geno.1998.5733. PMID 10191091.
^ Gustincich S, Schneider C (septembar 1993). "Serum deprivation response gene is induced by serum starvation but not by contact inhibition". Cell Growth & Differentiation. 4 (9): 753–60. PMID 8241023.
^ ^a ^b "Entrez Gene: SDPR serum deprivation response (phosphatidylserine binding protein)".
^ ^a ^b ^c ^d ^e Boopathy GT, Kulkarni M, Ho SY, Boey A, Chua EW, Barathi VA, et al. (oktobar 2017). "Cavin-2 regulates the activity and stability of endothelial nitric-oxide synthase (eNOS) in angiogenesis". The Journal of Biological Chemistry. 292 (43): 17760–17776. doi:10.1074/jbc.M117.794743. PMC 5663877. PMID 28912276.
^ "UniProt, O95810" (jezik: англ.). Pristupljeno 19. 11. 2021.CS1 održavanje: nepoznati jezik (link)
^ ^a ^b ^c ^d Ozturk S, Papageorgis P, Wong CK, Lambert AW, Abdolmaleky HM, Thiagalingam A, et al. (januar 2016). "SDPR functions as a metastasis suppressor in breast cancer by promoting apoptosis". Proceedings of the National Academy of Sciences of the United States of America. 113 (3): 638–43. Bibcode:2016PNAS..113..638O. doi:10.1073/pnas.1514663113. PMC 4725521. PMID 26739564.

Literatura

Burgener R, Wolf M, Ganz T, Baggiolini M (august 1990). "Purification and characterization of a major phosphatidylserine-binding phosphoprotein from human platelets". The Biochemical Journal. 269 (3): 729–34. doi:10.1042/bj2690729. PMC 1131648. PMID 2390065.
Cross SH, Charlton JA, Nan X, Bird AP (mart 1994). "Purification of CpG islands using a methylated DNA binding column". Nature Genetics. 6 (3): 236–44. doi:10.1038/ng0394-236. PMID 8012384. S2CID 12847618.
Mineo C, Ying YS, Chapline C, Jaken S, Anderson RG (maj 1998). "Targeting of protein kinase Calpha to caveolae". The Journal of Cell Biology. 141 (3): 601–10. doi:10.1083/jcb.141.3.601. PMC 2132740. PMID 9566962.
Gevaert K, Goethals M, Martens L, Van Damme J, Staes A, Thomas GR, Vandekerckhove J (maj 2003). "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides". Nature Biotechnology. 21 (5): 566–9. doi:10.1038/nbt810. PMID 12665801. S2CID 23783563.
Brandenberger R, Wei H, Zhang S, Lei S, Murage J, Fisk GJ, et al. (juni 2004). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nature Biotechnology. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197. S2CID 27764390.
Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al. (oktobar 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000168497 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000045954 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid10191091-5] Gustincich S, Vatta P, Goruppi S, Wolf M, Saccone S, Della Valle G, et al. (april 1999). "The human serum deprivation response gene (SDPR) maps to 2q32-q33 and codes for a phosphatidylserine-binding protein". Genomics. 57 (1): 120–9. doi:10.1006/geno.1998.5733. PMID 10191091.

[pmid8241023-6] Gustincich S, Schneider C (septembar 1993). "Serum deprivation response gene is induced by serum starvation but not by contact inhibition". Cell Growth & Differentiation. 4 (9): 753–60. PMID 8241023.

[entrez-7] "Entrez Gene: SDPR serum deprivation response (phosphatidylserine binding protein)".

[Boopathy_2017-8] Boopathy GT, Kulkarni M, Ho SY, Boey A, Chua EW, Barathi VA, et al. (oktobar 2017). "Cavin-2 regulates the activity and stability of endothelial nitric-oxide synthase (eNOS) in angiogenesis". The Journal of Biological Chemistry. 292 (43): 17760–17776. doi:10.1074/jbc.M117.794743. PMC 5663877. PMID 28912276.

[UniProt-9] "UniProt, O95810" (jezik: англ.). Pristupljeno 19. 11. 2021.CS1 održavanje: nepoznati jezik (link)

[pmid26739564-10] Ozturk S, Papageorgis P, Wong CK, Lambert AW, Abdolmaleky HM, Thiagalingam A, et al. (januar 2016). "SDPR functions as a metastasis suppressor in breast cancer by promoting apoptosis". Proceedings of the National Academy of Sciences of the United States of America. 113 (3): 638–43. Bibcode:2016PNAS..113..638O. doi:10.1073/pnas.1514663113. PMC 4725521. PMID 26739564.

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