SNAPC5

SNAPC5
Identifikatori
Aliasi	SNAPC5
Vanjski ID-jevi	OMIM: 605979 MGI: 1914282 HomoloGene: 4414 GeneCards: SNAPC5
Lokacija gena (čovjek) Hrom. Hromosom 15 (čovjek)[1] Bend 15q22.31 Početak 66,490,135 bp[1] Kraj 66,497,780 bp[1]
Lokacija gena (miš) Hrom. Hromosom 9 (miš)[2] Bend 9|9 C Početak 64,086,556 bp[2] Kraj 64,090,414 bp[2]
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • GO:0001131, GO:0001151, GO:0001130, GO:0001204 DNA-binding transcription factor activity • GO:0001948, GO:0016582 vezivanje za proteine Ćelijska komponenta • Jedarce • jedro • nukleoplazma • nuclear body Biološki proces • GO:0009373 regulation of transcription, DNA-templated • transcription by RNA polymerase II • transcription initiation from RNA polymerase III promoter • transcription, DNA-templated • snRNA transcription by RNA polymerase II Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	10302	330959
Ensembl	ENSG00000174446	ENSMUSG00000032398
UniProt	O75971	Q8R2K7
RefSeq (mRNK)	NM_001329613 NM_001329614 NM_001329615 NM_006049	NM_183316 NM_025925
RefSeq (bjelančevina)	NP_001316542 NP_001316543 NP_001316544 NP_006040	NP_899139
Lokacija (UCSC)	Chr 15: 66.49 – 66.5 Mb	Chr 9: 64.09 – 64.09 Mb
PubMed pretraga	[3]	[4]
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Podjedinica 5 snRNK-aktivirajućeg proteinskog komplesa je protein koji je kod ljudi kodiran genom SNAPC5.^[5][6]

Gross (2014) locirao je gen SNAPC5 na hromosomu 15q22.31, na osnovu poravnanja SNAPC5 sekvence (GenBank AF093593) sa genomskom sekvencom (GRCh37^[7]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 98 aminokiselina, a molekulska težina 11.328 Da.^[8]

10		20		30		40		50
MLSRLQELRK		EEETLLRLKA		ALHDQLNRLK		VEELALQSMI		SSRRGDEMLS
SHTVPEQSHD		MLVHVDNEAS		INQTTLELST		KSHVTEEEEE		EEEEESDS

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

SNAPC5 kodira podjedinicu snRNK-aktivirajućeg proteinskog kompleksa (SNAPc), koja je potrebna za transkripciju i RNK-polimeraze II i III snRNK (SNAPC1) (Henry et al., 1998).

Kloniranje i ekspresija

Analizom mikrosekvence 19-kD polipeptida prisutnog u SNAPC-u, praćenom pretragom baze podataka EST i RT-PCR analizom, Henry et al. (1998) dobili su cDNK za kodiranje SNAPC5, koju su nazvali SNAP19. Analiza sekvence predvidjela je da protein od 98 aminokiselina sadrži motiv leucinskog zatvarača na svom N-kraju i proteinski dio glutamata od 10 ostataka na C-kraju.

Funkcija gena

Koristeći superpromjenu i imunoblot analize, Henry et al. (1998) potvrdili su postojanje SNAPC5 u kompleksu s elementom proksimalne sekvence (PSE) promotora snRNK i da je potreban za transkripciju gena snRNK i pol II i pol III. Analiza imunoprecipitacije kotransliranih SNAP gena sugerira da SNAPC4 čini okosnicu na kojoj se okupljaju SNAPC2 i SNAPC5. Ovo potonje je neophodno za naknadno sastavljanje SNAPC1 sa SNAPC4, nakon čega slijedi sastavljanje SNAPC3 sa SNAPC1. Rekonstituirali su SNAPC iz pet rekombinantnih podjedinica i pokazali da se ovaj jedarnii kompleks veže za PSE i usmjerava transkripciju s RNK pol II i pol III snRNA.^[9]

Reference

1. GRCh38: Ensembl release 89: ENSG00000174446 - Ensembl, maj 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000032398 - Ensembl, maj 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Henry RW, Mittal V, Ma B, Kobayashi R, Hernandez N (Oct 1998). "SNAP19 mediates the assembly of a functional core promoter complex (SNAPc) shared by RNA polymerases II and III". Genes Dev. 12 (17): 2664–72. doi:10.1101/gad.12.17.2664. PMC 317148. PMID 9732265.
6. "Entrez Gene: SNAPC5 small nuclear RNA activating complex, polypeptide 5, 19kDa".
7. Gross, M. B. Personal Communication. Baltimore, Md. 4/16/2014.
8. "UniProt, O75971". Pristupljeno 18. 7. 2021.
9. Henry, R. W., Mittal, V., Ma, B., Kobayashi, R., Hernandez, N. SNAP19 mediates the assembly of a functional core promoter complex (SNAPc) shared by RNA polymerases II and III. Genes Dev. 12: 2664-2672, 1998. PubMed: 9732265

Dopunska literatura

• Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Brandenberger R, Wei H, Zhang S, et al. (2005). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nat. Biotechnol. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Ma B, Hernandez N (2001). "A map of protein-protein contacts within the small nuclear RNA-activating protein complex SNAPc". J. Biol. Chem. 276 (7): 5027–35. doi:10.1074/jbc.M009301200. PMID 11056176.

Vanjski linkovi

• SNAPC5 lokacija ljudskog genoma UCSC Genome Browser.
• Šablon:UCSC