USP1

USP1
Identifikatori
Aliasi	USP1
Vanjski ID-jevi	OMIM: 603478 MGI: 2385198 HomoloGene: 2528 GeneCards: USP1
Lokacija gena (čovjek) Hrom. Hromosom 1 (čovjek)[1] Bend 1p31.3 Početak 62,436,297 bp[1] Kraj 62,451,804 bp[1]
Lokacija gena (miš) Hrom. Hromosom 4 (miš)[2] Bend 4|4 C6 Početak 98,812,047 bp[2] Kraj 98,823,780 bp[2]
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • cysteine-type peptidase activity • GO:0001948, GO:0016582 vezivanje za proteine • hydrolase activity • thiol-dependent deubiquitinase • cysteine-type endopeptidase activity • GO:0070122 peptidase activity Ćelijska komponenta • jedro • nukleoplazma Biološki proces • skeletal system development • monoubiquitinated protein deubiquitination • response to UV • ubiquitin-dependent protein catabolic process • Proteoliza • cellular response to DNA damage stimulus • protein deubiquitination • GO:0100026 Popravka DNK • regulation of DNA repair • interstrand cross-link repair Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	7398	230484
Ensembl	ENSG00000162607	ENSMUSG00000028560
UniProt	O94782	Q8BJQ2
RefSeq (mRNK)	NM_003368 NM_001017415 NM_001017416	NM_001301414 NM_146144 NM_001356424
RefSeq (bjelančevina)	NP_001017415 NP_001017416 NP_003359	NP_001288343 NP_666256 NP_001343353
Lokacija (UCSC)	Chr 1: 62.44 – 62.45 Mb	Chr 4: 98.81 – 98.82 Mb
PubMed pretraga	[3]	[4]
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Ubikvitin karboksil-terminalna hidrolaza 1 jest enzim koji je kod ljudi kodiran genom USP1 sa hromosoma 1.^[5][6]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 785 aminokiselina, a molekulska težina 88.207 Da.^[7].

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

10		20		30		40		50
MPGVIPSESN		GLSRGSPSKK		NRLSLKFFQK		KETKRALDFT		DSQENEEKAS
EYRASEIDQV		VPAAQSSPIN		CEKRENLLPF		VGLNNLGNTC		YLNSILQVLY
FCPGFKSGVK		HLFNIISRKK		EALKDEANQK		DKGNCKEDSL		ASYELICSLQ
SLIISVEQLQ		ASFLLNPEKY		TDELATQPRR		LLNTLRELNP		MYEGYLQHDA
QEVLQCILGN		IQETCQLLKK		EEVKNVAELP		TKVEEIPHPK		EEMNGINSIE
MDSMRHSEDF		KEKLPKGNGK		RKSDTEFGNM		KKKVKLSKEH		QSLEENQRQT
RSKRKATSDT		LESPPKIIPK		YISENESPRP		SQKKSRVKIN		WLKSATKQPS
ILSKFCSLGK		ITTNQGVKGQ		SKENECDPEE		DLGKCESDNT		TNGCGLESPG
NTVTPVNVNE		VKPINKGEEQ		IGFELVEKLF		QGQLVLRTRC		LECESLTERR
EDFQDISVPV		QEDELSKVEE		SSEISPEPKT		EMKTLRWAIS		QFASVERIVG
EDKYFCENCH		HYTEAERSLL		FDKMPEVITI		HLKCFAASGL		EFDCYGGGLS
KINTPLLTPL		KLSLEEWSTK		PTNDSYGLFA		VVMHSGITIS		SGHYTASVKV
TDLNSLELDK		GNFVVDQMCE		IGKPEPLNEE		EARGVVENYN		DEEVSIRVGG
NTQPSKVLNK		KNVEAIGLLG		GQKSKADYEL		YNKASNPDKV		ASTAFAENRN
SETSDTTGTH		ESDRNKESSD		QTGINISGFE		NKISYVVQSL		KEYEGKWLLF
DDSEVKVTEE		KDFLNSLSPS		TSPTSTPYLL		FYKKL

Funkcija

Ovaj gen kodira člana porodice ubikvitin-specifične obrade (UBP) proteaza koji je deubikvitinirajući enzim (DUB) sa His i Cys domenima. Ovaj protein nalazi se u citoplazmi i cijepa ubikvitinski dio prekursora spojenih na ubikvitin i ubikvitiniliranih proteina.

Protein specifično deubikvitinira protein u putu popravke DNK Fanconijeve anemije (FA). Karakterizirana je alternativna transkripcija sa varijantama prerade.^[6]

Reference

1. GRCh38: Ensembl release 89: ENSG00000162607 - Ensembl, maj 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000028560 - Ensembl, maj 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Puente XS, Sanchez LM, Overall CM, Lopez-Otin C (Jul 2003). "Human and mouse proteases: a comparative genomic approach". Nat Rev Genet. 4 (7): 544–58. doi:10.1038/nrg1111. PMID 12838346. S2CID 2856065.
6. "Entrez Gene: USP1 ubiquitin specific peptidase 1".
7. "UniProt, O94782" (jezik: engleski). Pristupljeno 1. 12. 2021.

Dopunska literatura

• D'Andrea A, Pellman D (1999). "Deubiquitinating enzymes: a new class of biological regulators". Crit. Rev. Biochem. Mol. Biol. 33 (5): 337–52. doi:10.1080/10409239891204251. PMID 9827704.
• Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Fujiwara T, Saito A, Suzuki M, et al. (1999). "Identification and chromosomal assignment of USP1, a novel gene encoding a human ubiquitin-specific protease". Genomics. 54 (1): 155–8. doi:10.1006/geno.1998.5554. PMID 9806842.
• Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
• Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
• Nijman SM, Huang TT, Dirac AM, et al. (2005). "The deubiquitinating enzyme USP1 regulates the Fanconi anemia pathway". Mol. Cell. 17 (3): 331–9. doi:10.1016/j.molcel.2005.01.008. hdl:1874/17796. PMID 15694335.
• Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.

Vanjski linkovi