VPS45

VPS45
Identifikatori
Aliasi	VPS45
Vanjski ID-jevi	OMIM: 610035 MGI: 891965 HomoloGene: 5250 GeneCards: VPS45
Lokacija gena (čovjek) Hrom. Hromosom 1 (čovjek)[1] Bend 1q21.2 Početak 150,067,279 bp[1] Kraj 150,145,329 bp[1]
Lokacija gena (miš) Hrom. Hromosom 3 (miš)[2] Bend 3 F2.1|3 41.65 cM Početak 95,907,144 bp[2] Kraj 95,965,778 bp[2]
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • GO:0001948, GO:0016582 vezivanje za proteine • molekularna funkcija Ćelijska komponenta • integral component of membrane • endozom • Golđijev aparat • endosome membrane • membrana • Golđijeva membrana • ćelijska komponenta • Sinapsna vezikula Biološki proces • protein transport • intracellular protein transport • Koagulacija (krv) • vesicle-mediated transport • vesicle docking involved in exocytosis Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	11311	22365
Ensembl	ENSG00000136631	ENSMUSG00000015747
UniProt	Q9NRW7	P97390
RefSeq (mRNK)	NM_001279353 NM_001279354 NM_001279355 NM_007259	NM_013841
RefSeq (bjelančevina)	NP_001266282 NP_001266283 NP_009190	NP_038869
Lokacija (UCSC)	Chr 1: 150.07 – 150.15 Mb	Chr 3: 95.91 – 95.97 Mb
PubMed pretraga	[3]	[4]
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Vakuolski protein 45 pridružen sortiranju jest protein koji je kod ljudi kodiran genom VPS45 sa hromosoma 1.^[5][6]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 570 aminokiselina, a molekulska težina 65.077 Da.^[7]

• C: Cistein
• D: Asparaginska kiselina
• E: Glutaminska kiselina
• F: Fenilalanin
• G: Glicin
• H: Histidin
• I: Izoleucin
• K: Lizin
• L: Leucin
• M: Metionin
• N: Asparagin
• P: Prolin
• Q: Glutamin
• R: Arginin
• S: Serin
• T: Treonin
• V: Valin
• W: Triptofan
• Y: Tirozin

10		20		30		40		50
MNVVFAVKQY		ISKMIEDSGP		GMKVLLMDKE		TTGIVSMVYT		QSEILQKEVY
LFERIDSQNR		EIMKHLKAIC		FLRPTKENVD		YIIQELRRPK		YTIYFIYFSN
VISKSDVKSL		AEADEQEVVA		EVQEFYGDYI		AVNPHLFSLN		ILGCCQGRNW
DPAQLSRTTQ		GLTALLLSLK		KCPMIRYQLS		SEAAKRLAEC		VKQVITKEYE
LFEFRRTEVP		PLLLILDRCD		DAITPLLNQW		TYQAMVHELL		GINNNRIDLS
RVPGISKDLR		EVVLSAENDE		FYANNMYLNF		AEIGSNIKNL		MEDFQKKKPK
EQQKLESIAD		MKAFVENYPQ		FKKMSGTVSK		HVTVVGELSR		LVSERNLLEV
SEVEQELACQ		NDHSSALQNI		KRLLQNPKVT		EFDAARLVML		YALHYERHSS
NSLPGLMMDL		RNKGVSEKYR		KLVSAVVEYG		GKRVRGSDLF		SPKDAVAITK
QFLKGLKGVE		NVYTQHQPFL		HETLDHLIKG		RLKENLYPYL		GPSTLRDRPQ
DIIVFVIGGA		TYEEALTVYN		LNRTTPGVRI		VLGGTTVHNT		KSFLEEVLAS
GLHSRSKESS		QVTSRSASRR

Funkcija

Vezikulski posredovano sortiranje proteina ima važnu ulogu u segregaciji unutarćelijskihih molekula u različite organela. Genetičke studije kvasca identifikovale su više od 40 vakuokskih sortiranja proteina (VPS) gena uključenih u transport vezikula do vakuola. Ovaj gen je član porodice Sec1 domena i pokazuje visok stepen sličnosti sekvence sa mišjim, pacovskim i kvaščevim Vps45. Tačna funkcija ovog gena nije poznata, ali njegova visoka ekspresija u monjedarnim ćelijamama periferne krvi ukazuje na ulogu u prometu proteina, uključujući upalnene medijatore.^[6]

Reference

1. GRCh38: Ensembl release 89: ENSG00000136631 - Ensembl, maj 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000015747 - Ensembl, maj 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Pevsner J, Hsu SC, Hyde PS, Scheller RH (Feb 1997). "Mammalian homologues of yeast vacuolar protein sorting (vps) genes implicated in Golgi-to-lysosome trafficking". Gene. 183 (1–2): 7–14. doi:10.1016/S0378-1119(96)00367-8. PMID 8996080.
6. "Entrez Gene: VPS45 vacuolar protein sorting 45 homolog (S. cerevisiae)".
7. "UniProt, Q9NRW7" (jezik: en.). Pristupljeno 9. 12. 2021.

Dopunska literatura

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Tellam JT, James DE, Stevens TH, Piper RC (1997). "Identification of a mammalian Golgi Sec1p-like protein, mVps45". J. Biol. Chem. 272 (10): 6187–93. doi:10.1074/jbc.272.10.6187. PMID 9045632.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Rajasekariah P, Eyre HJ, Stanley KK, et al. (1999). "Molecular cloning and characterization of a cDNA encoding the human leucocyte vacuolar protein sorting (h1Vps45)". Int. J. Biochem. Cell Biol. 31 (6): 683–94. doi:10.1016/S1357-2725(99)00017-5. PMID 10404641.
• Nielsen E, Christoforidis S, Uttenweiler-Joseph S, et al. (2000). "Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain". J. Cell Biol. 151 (3): 601–12. doi:10.1083/jcb.151.3.601. PMC 2185588. PMID 11062261.
• de Renzis S, Sönnichsen B, Zerial M (2002). "Divalent Rab effectors regulate the sub-compartmental organization and sorting of early endosomes". Nat. Cell Biol. 4 (2): 124–33. doi:10.1038/ncb744. PMID 11788822. S2CID 6596498.
• Dulubova I, Yamaguchi T, Gao Y, et al. (2002). "How Tlg2p/syntaxin 16 'snares' Vps45". EMBO J. 21 (14): 3620–31. doi:10.1093/emboj/cdf381. PMC 126126. PMID 12110575.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Kim BY, Ueda M, Kominami E, et al. (2004). "Identification of mouse Vps16 and biochemical characterization of mammalian class C Vps complex". Biochem. Biophys. Res. Commun. 311 (3): 577–82. doi:10.1016/j.bbrc.2003.10.030. PMID 14623309.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.