ZC3H13

ZC3H13
Identifikatori
Aliasi	ZC3H13
Vanjski ID-jevi	OMIM: 616453 MGI: 1914552 GeneCards: ZC3H13
Lokacija gena (čovjek) Hrom. Hromosom 13 (čovjek)[1] Bend 13q14.13 Početak 45,954,465 bp[1] Kraj 46,052,759 bp[1]
Lokacija gena (miš) Hrom. Hromosom 14 (miš)[2] Bend 14|14 D3 Početak 75,521,813 bp[2] Kraj 75,581,866 bp[2]
Obrazac RNK ekspresije Više referentnih podataka o ekspresiji
Ontologija gena Molekularna funkcija • GO:0001948, GO:0016582 vezivanje za proteine • vezivanje iona metala • vezivanje sa RNK Ćelijska komponenta • nuclear speck • jedro • nukleoplazma • RNA N6-methyladenosine methyltransferase complex Biološki proces • mRNA processing • multicellular organism development • Prerada RNK • mRNA methylation • regulation of stem cell population maintenance Izvori:Amigo / QuickGO
Ortolozi
Vrste	Čovjek	Miš
Entrez	23091	67302
Ensembl	ENSG00000123200	ENSMUSG00000022000
UniProt	Q5T200	E9Q784
RefSeq (mRNK)	NM_015070 NM_001076788 NM_001330564 NM_001330565 NM_001330566 NM_001330567 NM_001382206 NM_001382207 NM_001382208 NM_001382209 NM_001382210 NM_001382211 NM_001382212 NM_001382213 NM_001382214	NM_026083 NM_027377 NM_001359875
RefSeq (bjelančevina)	NP_001070256 NP_001317493 NP_001317494 NP_001317495 NP_001317496 NP_055885 NP_001369135 NP_001369136 NP_001369137 NP_001369138 NP_001369139 NP_001369140 NP_001369141 NP_001369142 NP_001369143	NP_080359 NP_001346804
Lokacija (UCSC)	Chr 13: 45.95 – 46.05 Mb	Chr 14: 75.52 – 75.58 Mb
PubMed pretraga	[3]	[4]
Wikipodaci
Pogledaj/uredi – čovjek Pogledaj/uredi – miš

Protein 13 cinkovog prsta sa CCCH domenom je protein koji je kod ljudi kodiran genom ZC3H13, sa hromosoma 13, sekvenca q14:45,954.464 – 46,052,793 (prema NCBI) .^[5][6]

Aminokiselinska sekvenca

Dužina polipeptidnog lanca je 1.668 aminokiselina, a molekulska težina 196.635 Da.^[7].

10		20		30		40		50
MSKIRRKVTV		ENTKTISDST		SRRPSVFERL		GPSTGSTAET		QCRNWLKTGN
CLYGNTCRFV		HGPSPRGKGY		SSNYRRSPER		PTGDLRERMK		NKRQDVDTEP
QKRNTEESSS		PVRKESSRGR		HREKEDIKIT		KERTPESEEE		NVEWETNRDD
SDNGDINYDY		VHELSLEMKR		QKIQRELMKL		EQENMEKREE		IIIKKEVSPE
VVRSKLSPSP		SLRKSSKSPK		RKSSPKSSSA		SKKDRKTSAV		SSPLLDQQRN
SKTNQSKKKG		PRTPSPPPPI		PEDIALGKKY		KEKYKVKDRI		EEKTRDGKDR
GRDFERQREK		RDKPRSTSPA		GQHHSPISSR		HHSSSSQSGS		SIQRHSPSPR
RKRTPSPSYQ		RTLTPPLRRS		ASPYPSHSLS		SPQRKQSPPR		HRSPMREKGR
HDHERTSQSH		DRRHERREDT		RGKRDREKDS		REEREYEQDQ		SSSRDHRDDR
EPRDGRDRRD		ARDTRDRREL		RDSRDMRDSR		EMRDYSRDTK		ESRDPRDSRS
TRDAHDYRDR		EGRDTHRKED		TYPEESRSYG		RNHLREESSR		TEIRNESRNE
SRSEIRNDRM		GRSRGRVPEL		PEKGSRGSRG		SQIDSHSSNS		NYHDSWETRS
SYPERDRYPE		RDNRDQARDS		SFERRHGERD		RRDNRERDQR		PSSPIRHQGR
NDELERDERR		EERRVDRVDD		RRDERARERD		RERERDRERE		RERERERDRE
REKERELERE		RARERERERE		KERDRERDRD		RDHDRERERE		RERDREKERE
REREERERER		ERERERERER		ERERERARER		DKERERQRDW		EDKDKGRDDR
REKREEIRED		RNPRDGHDER		KSKKRYRNEG		SPSPRQSPKR		RREHSPDSDA
YNSGDDKNEK		HRLLSQVVRP		QESRSLSPSH		LTEDRQGRWK		EEDRKPERKE
SSRRYEEQEL		KEKVSSVDKQ		REQTEILESS		RMRAQDIIGH		HQSEDRETSD
RAHDENKKKA		KIQKKPIKKK		KEDDVGIERG		NIETTSEDGQ		VFSPKKGQKK
KSIEKKRKKS		KGDSDISDEE		AAQQSKKKRG		PRTPPITTKE		ELVEMCNGKN
GILEDSQKKE		DTAFSDWSDE		DVPDRTEVTE		AEHTATATTP		GSTPSPLSSL
LPPPPPVATA		TATTVPATLA		ATTAAAATSF		STSAITISTS		ATPTNTTNNT
FANEDSHRKC		HRTRVEKVET		PHVTIEDAQH		RKPMDQKRSS		SLGSNRSNRS
HTSGRLRSPS		NDSAHRSGDD		QSGRKRVLHS		GSRDREKTKS		LEITGERKSR
IDQLKRGEPS		RSTSSDRQDS		RSHSSRRSSP		ESDRQVHSRS		GSFDSRDRLQ
ERDRYEHDRE		RERERRDTRQ		REWDRDADKD		WPRNRDRDRL		RERERERERD
KRRDLDRERE		RLISDSVERD		RDRDRDRTFE		SSQIESVKRC		EAKLEGEHER
DLESTSRDSL		ALDKERMDKD		LGSVQGFEET		NKSERTESLE		GDDESKLDDA
HSLGSGAGEG		YEPISDDELD		EILAGDAEKR		EDQQDEEKMP		DPLDVIDVDW
SGLMPKHPKE		PREPGAALLK		FTPGAVMLRV		GISKKLAGSE		LFAKVKETCQ
RLLEKPKDAD		NLFEHELGAL		NMAALLRKEE		RASLLSNLGP		CCKALCFRRD
SAIRKQLVKN		EKGTIKQAYT		SAPMVDNELL		RLSLRLFKRK		TTCHAPGHEK
TEDNKLSQSS		IQQELCVS

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

Kloniranje i ekspresija

Sekvenciranjem klonova dobijenih iz biblioteke cDNK ljudskog mozga frakcionirane veličine, Nagase et al. (1998) klonirali su ZC3H13, koji su označili kao KIAA0853. Izvedeni protein od 967 aminokiselina ima slabu homologiju s ljudskim trihohialinom (TCHH). RT-PCR ELISA testom otkrili je najjaču ekspresiju KIAA0853 u jetri, jajnicima i mozgu, zatim u bubrezima. Umjerena ekspresija otkrivena je u srcu, plućima, skeletnim mišićima, gušterači i sjemenicima.^[8]

Funkcija gena

Koristeći masenu spektrometrijsku analizu za identifikaciju proteina koji su imunoprecipitirani sa WTAP iz endotelnih ćelija ljudske pupčane vene (HUVEC), Horiuchi et al. (2013) identificirali su proteinski kompleks, koji je uključivao KIAA0853, HAKAI (CBLL1), virilizer (KIAA1429), RBM15, BCLAF1 i THRAP3. WTAP kompleks lokaliziran na jedarnim pjegama HeLa ćelija i HUVEC-ovima, a eksperimenti umrežavanja pokazali su da je prolazno povezan s faktorima prerade. Nokauniranje bilo koje komponente WTAP kompleksa, uključujući KIAA0853, smanjilo je alternativnu preradu WTAP-a, što je rezultiralo povećanom proizvodnjom proteina pune dužine WTAP-a i smanjenom proliferacijom ćelija, sa zastojem ćelijskog ciklusa u fazi G2. Razgradnja BCLAF1 i THRAP3 smanjila je lokalizaciju jedarnih pjega drugih komponenti WTAP kompleksa.^[9]

Reference

1. GRCh38: Ensembl release 89: ENSG00000123200 - Ensembl, maj 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000022000 - Ensembl, maj 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (maj 1999). "Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 5 (6): 355–64. doi:10.1093/dnares/5.6.355. PMID 10048485.
6. "Entrez Gene: ZC3H13 zinc finger CCCH-type containing 13".
7. "UniProt, Q5T200". Pristupljeno 11. 9. 2017.
8. Nagase, T., Ishikawa, K., Suyama, M., Kikuno, R., Hirosawa, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Oharo, O. Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 5: 355-364, 1998. PubMed: 10048485
9. Horiuchi, K., Kawamura, T., Iwanari, H., Ohashi, R., Naito, M., Kodama, T., Hamakubo, T. Identification of Wilms' tumor 1-associating protein complex and its role in alternative splicing and the cell cycle. J. Biol. Chem. 288: 33292-33302, 2013. PubMed: 24100041

Dopunska literatura

• Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Obuse C, Yang H, Nozaki N, et al. (2004). "Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase". Genes Cells. 9 (2): 105–20. doi:10.1111/j.1365-2443.2004.00705.x. PMID 15009096.
• Dunham A, Matthews LH, Burton J, et al. (2004). "The DNA sequence and analysis of human chromosome 13". Nature. 428 (6982): 522–8. doi:10.1038/nature02379. PMC 2665288. PMID 15057823.
• Brill LM, Salomon AR, Ficarro SB, et al. (2004). "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry". Anal. Chem. 76 (10): 2763–72. doi:10.1021/ac035352d. PMID 15144186.
• Brandenberger R, Wei H, Zhang S, et al. (2005). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nat. Biotechnol. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID 16964243.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.