ZZZ3
Protein 3 sa ZZ-tipim cinkovog prsta jest protein koji je kod ljudi kodiran genom ZZZ3 sa hromosoma 1.[5]
Aminokiselinska sekvenca
[uredi | uredi izvor]Dužina polipeptidnog lanca je 903 aminokiseline, a molekulska težina 102.023 Da.[6]
| 10 | 20 | 30 | 40 | 50 | ||||
|---|---|---|---|---|---|---|---|---|
| MAASRSTRVT | RSTVGLNGLD | ESFCGRTLRN | RSIAHPEEIS | SNSQVRSRSP | ||||
| KKRPEPVPIQ | KGNNNGRTTD | LKQQSTRESW | VSPRKRGLSS | SEKDNIERQA | ||||
| IENCERRQTE | PVSPVLKRIK | RCLRSEAPNS | SEEDSPIKSD | KESVEQRSTV | ||||
| VDNDADFQGT | KRACRCLILD | DCEKREIKKV | NVSEEGPLNS | AVVEEITGYL | ||||
| AVNGVDDSDS | AVINCDDCQP | DGNTKQNSIG | SYVLQEKSVA | ENGDTDTQTS | ||||
| MFLDSRKEDS | YIDHKVPCTD | SQVQVKLEDH | KIVTACLPVE | HVNQLTTEPA | ||||
| TGPFSETQSS | LRDSEEEVDV | VGDSSASKEQ | CKENTNNELD | TSLESMPASG | ||||
| EPEPSPVLDC | VSAQMMSLSE | PQEHRYTLRT | SPRRAAPTRG | SPTKNSSPYR | ||||
| ENGQFEENNL | SPNETNATVS | DNVSQSPTNP | GEISQNEKGI | CCDSQNNGSE | ||||
| GVSKPPSEAR | LNIGHLPSAK | ESASQHITEE | EDDDPDVYYF | ESDHVALKHN | ||||
| KDYQRLLQTI | AVLEAQRSQA | VQDLESLGRH | QREALKNPIG | FVEKLQKKAD | ||||
| IGLPYPQRVV | QLPEIVWDQY | THSLGNFERE | FKNRKRHTRR | VKLVFDKVGL | ||||
| PARPKSPLDP | KKDGESLSYS | MLPLSDGPEG | SSSRPQMIRG | RLCDDTKPET | ||||
| FNQLWTVEEQ | KKLEQLLIKY | PPEEVESRRW | QKIADELGNR | TAKQVASRVQ | ||||
| KYFIKLTKAG | IPVPGRTPNL | YIYSKKSSTS | RRQHPLNKHL | FKPSTFMTSH | ||||
| EPPVYMDEDD | DRSCFHSHMN | TAVEDASDDE | SIPIMYRNLP | EYKELLQFKK | ||||
| LKKQKLQQMQ | AESGFVQHVG | FKCDNCGIEP | IQGVRWHCQD | CPPEMSLDFC | ||||
| DSCSDCLHET | DIHKEDHQLE | PIYRSETFLD | RDYCVSQGTS | YNYLDPNYFP | ||||
| ANR |
Modelni organizmi
[uredi | uredi izvor]| Svojstvo | Fenotip |
|---|---|
| Vijabilnost homozigota | Nenormalnan |
| Studija recesivne letalnosti | Nenormalnan |
| Tjeelsna težina | Normalan |
| Anksioznost otvorenog polja | Normalan |
| Neurološka peocjena | Normalan |
| Snaga stiska | Normalan |
| Test vruće ploče | Normalan |
| Dismorfologija | Normalan |
| Indirektna kalorimetrija | Normalan |
| Test tolerancije glukoze | Normalan |
| Slušni odgovor moždanog stabla | Normalan |
| DEXA | Normalan |
| Radiografija | Normalan |
| Tjelesna temperatura | Normalan |
| Morfologija oka | Normalan |
| Klinička hemija | Normalan |
| Hematologija | Normalan |
| Limfociti periferne krvi | Normalan |
| Mikronukleus test | Normalan |
| Težina srca | Normalan |
| Tail epidermis wholemount | Normalan |
| Histopatologija kože | Normalan |
| Histopatologija mozga | Normalan |
| Citrobacter infekcija | Normalan[7] |
| Svi testovi I analize iz[8][9] |
U proučavanju funkcije ZZZ3 korišteni su modelni organizmi. Uvjetna linija nokaut-miševa, zvana Zzz3tm1a(EUCOMM)Wtsi [10][11] generirana je kao dio programa Međunarodnog konzorcija za nokaut-miševe — projekta mutageneze visoke propusnosti za generiranje i distribuciju životinjskih modela bolesti zainteresiranim naučnicima.[12][13][14]
Mužjaci i ženke podvrgnute su standardiziranom fenotipskom pregledu, kako bi se utvrdili efekti delecija.[8][15] Obavljena su 24 testa na mutantnim miševima i uočene su dvije značajne abnormalnosti.[8] Nijedan homozigotni mutantni embrion nije identificiran tokom gestacije, pa stoga nijedan nije preživio do odbijanja. Preostali testovi su sprovedeni na heterozigot nim mutantnim odraslim miševima i nisu identifikovani dalji abnormalni fenotipovi.[8]
Također pogledajte
[uredi | uredi izvor]Reference
[uredi | uredi izvor]- ^ a b c GRCh38: Ensembl release 89: ENSG00000036549 - Ensembl, maj 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000039068 - Ensembl, maj 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Entrez Gene: zinc finger, ZZ-type containing 3". Pristupljeno 30. 8. 2011.
- ^ "UniProt, Q8IYH5" (jezik: eng.). Pristupljeno 26. 11. 2021.CS1 održavanje: nepoznati jezik (link)
- ^ "Citrobacter infection data for Zzz3". Wellcome Trust Sanger Institute.
- ^ a b c d Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica. 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. S2CID 85911512.
- ^ Mouse Resources Portal, Wellcome Trust Sanger Institute.
- ^ "International Knockout Mouse Consortium". Arhivirano s originala, 3. 4. 2012. Pristupljeno 26. 11. 2021.
- ^ "Mouse Genome Informatics".
- ^ Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
- ^ Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
- ^ Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. S2CID 18872015.
- ^ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
Dopunska literatura
[uredi | uredi izvor]- Guelman S, Suganuma T, Florens L, Swanson SK, Kiesecker CL, Kusch T, Anderson S, Yates JR, Washburn MP, Abmayr SM, Workman JL (Feb 2006). "Host cell factor and an uncharacterized SANT domain protein are stable components of ATAC, a novel dAda2A/dGcn5-containing histone acetyltransferase complex in Drosophila". Molecular and Cellular Biology. 26 (3): 871–82. doi:10.1128/MCB.26.3.871-882.2006. PMC 1347012. PMID 16428443.
- Ravasi T, Suzuki H, Cannistraci CV, Katayama S, Bajic VB, Tan K, Akalin A, Schmeier S, Kanamori-Katayama M, Bertin N, Carninci P, Daub CO, Forrest AR, Gough J, Grimmond S, Han JH, Hashimoto T, Hide W, Hofmann O, Kamburov A, Kaur M, Kawaji H, Kubosaki A, Lassmann T, van Nimwegen E, MacPherson CR, Ogawa C, Radovanovic A, Schwartz A, Teasdale RD, Tegnér J, Lenhard B, Teichmann SA, Arakawa T, Ninomiya N, Murakami K, Tagami M, Fukuda S, Imamura K, Kai C, Ishihara R, Kitazume Y, Kawai J, Hume DA, Ideker T, Hayashizaki Y (Mar 2010). "An atlas of combinatorial transcriptional regulation in mouse and man". Cell. 140 (5): 744–52. doi:10.1016/j.cell.2010.01.044. PMC 2836267. PMID 20211142.